血管内皮祖细胞对共植入缺血再灌注模型大鼠神经干细胞增殖、凋亡和血管重建的影响

发布时间：2018-06-08 01:45

本文选题：再灌注损伤 + 神经干细胞　；参考：《中国组织工程研究》2017年05期

【摘要】：背景:神经干细胞移植在缺血性脑血管疾病治疗中的作用已经明确,但细胞不能在移植局部存活等问题限制了进一步的应用。目的:探讨血管内皮祖细胞对共植入缺血再灌注模型大鼠神经干细胞增殖、凋亡和血管重建的影响。方法:125只SD大鼠随机分为5组,假手术组、缺血对照组、神经干细胞组、神经干细胞+血管内皮祖细胞组、血管内皮祖细胞组,每组25只。线栓法制备缺血再灌注大鼠模型,并进行分组干预。移植后1,2,3,4,5周,检测各组神经干细胞增殖、凋亡以及缺血区血管新生情况。结果与结论:(1)移植后不同时间点,血管内皮祖细胞组、缺血对照组、假手术组均未观察到BrdU阳性细胞。神经干细胞+血管内皮祖细胞组的Brd U阳性细胞数显著高于神经干细胞(P0.05);(2)移植后不同时间点,神经干细胞+血管内皮祖细胞组和神经干细胞组可观察到BrdU+/Caspase-3+双标阳性细胞。神经干细胞+血管内皮祖细胞组的细胞凋亡率显著低于神经干细胞组(P0.05);(3)移植后不同时间点,假手术组均未见新生血管,其他4组均出现新生血管。神经干细胞+血管内皮祖细胞组的血管新生数量显著多于其他各组(P0.05);(4)结果表明,血管内皮祖细胞可以促进共植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖,抑制细胞凋亡,并促进缺血区血管新生。
[Abstract]:Background: the role of neural stem cell transplantation in the treatment of ischemic cerebrovascular diseases has been clear, but the problem that cells can not survive in the local graft limits the further application. Aim: to investigate the effects of vascular endothelial progenitor cells (EPCs) on the proliferation, apoptosis and vascular remodeling of neural stem cells (NSCs) implanted into ischemia reperfusion model rats. Methods 125 Sprague-Dawley rats were randomly divided into 5 groups: sham operation group, ischemic control group and neural stem cell endothelial progenitor cell group (25 rats in each group). The rat model of ischemia reperfusion was established by the method of thread embolization, and intervention was carried out in groups. The proliferation and apoptosis of neural stem cells and angiogenesis in ischemic area were detected at 5 weeks after transplantation. Results and conclusion BrdU positive cells were not observed in vascular endothelial progenitor cells group ischemia control group and sham operation group at different time points after transplantation. The number of BrdU positive cells in neural stem cell vascular endothelial progenitor cell group was significantly higher than that in neural stem cell group (P0.05P0.05P0.05 + 2). BrdU / Caspase-3 double labeled positive cells were observed in neural stem cell vascular endothelial progenitor cell group and neural stem cell group at different time points after transplantation. The apoptotic rate of neural stem cell endothelial progenitor cells group was significantly lower than that of neural stem cell group (P 0.05). No neovascularization was found in the sham operation group, while neovascularization was found in the other four groups. The number of neovascularization in neural stem cell endothelial progenitor cells group was significantly higher than that in other groups (P 0.05). The results showed that vascular endothelial progenitor cells could promote the proliferation of neural stem cells implanted into ischemic penumbra of rats. Inhibit apoptosis and promote angiogenesis in ischemic area.
【作者单位】：新乡医学院第三附属医院神经内科;
【分类号】：R-332;R743

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