甲状旁腺激素对大鼠骨髓间充质干细胞钙离子的影响

发布时间：2018-06-09 17:32

本文选题：间质干细胞 + 甲状旁腺激素肽(-)　；参考：《中国组织工程研究》2017年29期

【摘要】：背景:甲状旁腺激素可通过靶器官表面的特异性受体激活一系列生理生化反应,调节钙的动态平衡。目的:观察甲状旁腺激素对骨髓间充质干细胞内钙离子的影响,探讨其维持骨髓间充质干细胞体外增殖的机制。方法:将第4代SD大鼠骨髓间充质干细胞分组培养,实验组加入含甲状旁腺激素的培养基,对照组加入常规培养基,培养10 d内,MTT法检测细胞增殖;培养7,14 d,通过激光共聚焦显微镜观察实验组及对照组细胞内钙离子荧光峰值。结果与结论:①细胞增殖:培养第一二天,2组细胞生长活性均滞缓,3 d后进入快速增长期,在第七八天进入平台期,第10天细胞增殖能力有减弱趋势,其中对照组细胞增殖能力低于实验组(P0.05);②激光共聚焦显微镜观察结果:两组于培养第7天可见胞内呈现较强的钙荧光峰值,随着诱导时间延长,14 d的细胞内钙荧光峰值出现下降,实验组培养7,14 d的细胞内的钙荧光峰值强于对照组(P0.001),且实验组14 d的细胞内钙荧光峰值与对照组7 d的细胞内钙荧光峰值无差异;③结果表明:甲状旁腺激素可增加骨髓间充质干细胞内钙离子荧光峰值,维持骨髓间充质干细胞增殖功能。
[Abstract]:Background: parathyroid hormone (PTH) can activate a series of physiological and biochemical reactions through specific receptors on target organ surface and regulate the dynamic balance of calcium. Aim: to observe the effect of parathyroid hormone (PTH) on calcium in bone marrow mesenchymal stem cells (BMSCs) and to explore the mechanism of maintaining the proliferation of BMSCs in vitro. Methods: bone marrow mesenchymal stem cells (BMSCs) of the 4th generation SD rats were cultured in groups. The experimental group was added with parathyroid hormone medium, the control group was added to the conventional culture medium, and the proliferation of the cells was detected by MTT assay within 10 days. The peak of intracellular calcium fluorescence was observed by laser confocal microscope for 7d and 14d in the experimental group and control group. Results and conclusion: the growth activity of the cells in the first and second day of culture of the two groups was retarded for 3 days, then entered into the stage of rapid growth on the 7th and 8th day, and decreased on the 10th day. The proliferative ability of the control group was lower than that of the experimental group. The results of laser confocal microscopy showed that the intracellular calcium fluorescence peak was higher in the two groups on the 7th day of culture. The peak value of intracellular calcium fluorescence decreased with the prolongation of induction time for 14 days. The peak value of intracellular calcium fluorescence in the experimental group was stronger than that in the control group on the 14th day, and there was no difference between the experimental group on the 14th day and the control group on the 7th day. The results showed that parathyroid hormone could increase the peak of calcium fluorescence in bone marrow mesenchymal stem cells and maintain the proliferation function of bone marrow mesenchymal stem cells.
【作者单位】：广州医科大学附属第一医院广东省骨科矫形与植入材料重点实验室;南方医科大学顺德医院骨科;
【分类号】：Q813.11;R-33

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