人脐带间充质干细胞来源的外泌体免疫调节功能异质性研究

发布时间：2018-06-10 14:37

本文选题：外泌体 + 间充质干细胞　；参考：《军事医学》2017年06期

【摘要】：目的研究不同供者来源的人脐带间充质干细胞(h UC-MSC)分泌的外泌体免疫调节能力的异质性。方法培养5株不同供者来源的h UC-MSC,收集无血清培养上清提取外泌体,流式细胞术检测其表面特异性标志CD9、CD63、CD81、CD44的表达,BCA法测定总蛋白含量。将外泌体和脐带血单个核细胞(UBMC)共培养72 h,MTT实验检测细胞增殖活性,ELISA测定共培养上清中干扰素γ(IFN-γ)和肿瘤坏死因子α(TNF-α)的含量。离心重悬UBMC和K562细胞按5∶1的比例共培养4 h,MTT实验检测细胞活性,计算UBMC的杀伤能力。结果提取的外泌体表达CD9,CD63,CD81及CD44。不同供者来源h UC-MSC分泌的外泌体对UBMC的增殖、分泌细胞因子及K562细胞杀伤能力的作用不同。结论不同供者h UC-MSC的外泌体免疫调节能力具有异质性。
[Abstract]:Objective to study the heterogeneity of the immune regulatory capacity of human umbilical cord mesenchymal stem cells secreted by human umbilical cord mesenchymal stem cells (hUC-MSCs) from different donors. Methods five strains of hUC-MSCs from different donor sources were cultured, and the exocrine was extracted from the supernatant of serum-free culture. The expression of CD9mCD63, CD81 and CD44 was detected by flow cytometry and the total protein content was determined by BCA. UBMCs were co-cultured in exocrine and cord blood for 72 h. MTT assay was used to detect the proliferation activity of UBMCs. The levels of IFN- 纬) and TNF- 伪 in the co-culture supernatant were determined by Elisa. UBMC and K562 cells were cultured at 5:1 ratio for 4 h, and the cytotoxicity of UBMC was calculated by MTT assay. Results the exocrine bodies expressed CD9, CD63, CD81 and CD44. The exocrine secreted by hUC-MSC from different donors had different effects on UBMC proliferation, cytokine secretion and K562 cell killing ability. Conclusion the immunomodulation ability of hUC-MSC from different donors is heterogeneity.
【作者单位】：河北北方学院;空军总医院血液科;军事医学科学院放射与辐射医学研究所;
【基金】：首都卫生发展科研专项资助项目(2014-2-5122)
【分类号】：R329.2

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