异常黑胆质证2型糖尿病大鼠模型的血浆代谢组学研究

发布时间：2018-06-11 11:13

本文选题：异常黑胆质证 + 型糖尿病　；参考：《中国比较医学杂志》2017年08期

【摘要】：目的建立与分析2型糖尿病(T2DM)维吾尔医异常炓胆质证大鼠模型血浆代谢组学变化特点。方法建立T2DM维吾尔医异常黑胆质证大鼠模型并采集血浆样本,测试核磁共振氢谱,对数据进行多变量分析。结果单纯的异常黑胆质证组大鼠血浆中葡萄糖和乳酸含量下降的同时VLDL、LDL和肉碱的含量明显增加(P0.05);病证结合的T2DM维吾尔医异常黑胆质证组大鼠血浆中多种氨基酸(丙氨酸、异亮氨酸、、缬氨酸、亮氨酸、酪氨酸、谷氨酸、苯丙氨酸、甲基组氨酸、甘氨酸)以及乳酸、丙酮酸、糖蛋白、柠檬酸、肌酸酐、β-羟丁酸、乙酰乙酸、乙酸、肌酸、肉碱、鲨肌醇、VLDL、LDL的含量均降低,而葡萄糖和牛磺酸含量明显增加(P0.05)。结论蛋白质代谢、苯丙氨酸和酪氨酸代谢通路、糖酵解通路和脂代谢通路在病证结合的T2DM维吾尔医异常黑胆质证模型大鼠体内发生了明显的变化;血浆代谢组学变化在一定程度上反映病证结合的T2DM维吾尔医异常黑胆质证大鼠模型体内发生的病理变化过程。
[Abstract]:Objective to establish and analyze the changes of plasma metabolism in type 2 diabetes mellitus (T2DM) rats with abnormal gallbladder syndrome in Uygur medicine. Methods A rat model of T2DM with abnormal black gallbladder syndrome was established and plasma samples were collected. Nuclear magnetic resonance spectroscopy (NMR) was used to analyze the data. Results the contents of glucose and lactic acid in the plasma of the rats with simple abnormal black gallbladder syndrome decreased, while the contents of VLDL- LDL and carnitine increased significantly, while the levels of various amino acids (alanine, alanine) in plasma of T2DM Uygur Uygur patients with abnormal black gallbladder syndrome combined with disease and syndromes were significantly increased. Isoleucine, valine, leucine, tyrosine, glutamic acid, phenylalanine, methylhistidine, glycine) and lactic acid, pyruvate, glycoprotein, citric acid, creatinine, 尾 -hydroxybutyric acid, acetoacetic acid, creatine, carnitine, The contents of VLDL- LDL decreased, while the contents of glucose and taurine increased significantly (P 0.05). Conclusion protein metabolism, phenylalanine and tyrosine metabolism pathway, glycolysis pathway and lipid metabolism pathway have changed significantly in the model rats with abnormal black gallbladder syndrome of T2DM combined with T2DM. The changes of plasma metabolomics to some extent reflect the pathological changes in the model of abnormal black gallbladder syndrome of T2DM Uygur medicine combined with disease and syndromes.
【作者单位】：新疆医科大学药学院;新疆维吾尔自治区人民医院胰腺外科;新疆医科大学维吾尔医学院;新疆医科大学基础医学院;新疆医科大学中心实验室;
【基金】：国家自然科学基金(81460749)
【分类号】：R29;R-332

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