1～5月龄SPF级SD大鼠血液指标正常值范围及性别差异探讨

发布时间：2018-06-11 15:27

本文选题：SD大鼠 + 血液指标　；参考：《中药药理与临床》2017年01期

【摘要】：目的:建立1~5月龄无特定病原体(SPF)级SD大鼠血液指标正常范围,分析其性别差异,为实验动物结果分析提供实验数据参考。方法:选1至5月龄大鼠,取全血检测血液学指标、血清检测生化指标、血浆检测凝血酶原时间(PT)与活化部分凝血活酶时间(APTT)。将上述指标结果进行进行统计分析,计算95%置信区间,并比较相同月龄大鼠雌雄间性别差异。结果:1~5月龄大鼠PT、RBC、HGB、P-LCR,ALP、AST、BUN、CREA雌雄间差异皆有统计学意义,PT、RBC、HGB、P-LCR、ALP、AST雌性检测值低于雄性,BUN、CREA雌性检测值高于雄性;2~5月龄大鼠WBC、NEUT、MONO、LY、RET、ALT雌雄间差异有统计学意义,雌性检测值低于雄性;1月龄大鼠PLT雌雄间差异有统计学意义,雌性计数高于雄性;除2月龄外,1月龄、3~5月龄ALB雌雄间差异有统计学意义,雌性检测值高于雄性。RBC、WBC、HGB、ALP检测值随月龄增长呈上升趋势,LY、RET、CREA、ALB检测值随月龄增长呈下降趋势,在4至5月龄后,各项指标变化趋于平稳。结论:本研究建立本实验室1~5月龄SPF级SD大鼠血液指标正常值范围,结果提示,部分生化血液指标随月龄增长,有升高或降低,同一月龄大鼠,某些指标雌性检测水平会高于或低于雄性。
[Abstract]:Objective: to establish the normal range of blood indexes of 5 months old SD rats without specific pathogen, and to analyze the sex difference, and to provide experimental data for the analysis of experimental animal results. Methods: blood samples were collected from rats aged 1 to 5 months to detect hematological parameters, serum biochemical indexes, plasma prothrombin time (PTT) and activated partial thromboplastin time (APTTT). The 95% confidence interval was calculated and the sex difference between male and female rats of the same month was compared. Results there were significant differences between the male and female of the CREA of HGBP-LCRPER-ASTUNPERA (HGBP-LCRPMAN) of 5 month old rats. The detection value of the female was lower than that of the female of the male group of BUNCU, which was higher than that of the male rats of 25 months old. There was a significant difference between the male and the female of WBCNENNEUTMONONORLYRETRETATE (P < 0.01), and the difference of the female detection value between the male and the female group was statistically significant compared with that of the male group. The detection value of female was significantly lower than that of male rats at 1 month of age, and the female count was higher than that of male, and the difference of female and male between male and female was statistically significant, except for 2 months old and 1 month old and 5 months old ALB, there was a significant difference between male and female in PLT between male and female. The detection value of ALB in female was higher than that in male. The detection value of HGBP in female increased with the increase of age. The detection value of ALB decreased with the increase of age. After 4 to 5 months of age, the changes of the indexes tended to be stable. Conclusion: this study established the normal range of blood indexes of SPF SD rats of 1 ~ 5 months old in our laboratory. The results indicated that some biochemical blood indexes increased or decreased with the increase of age. Some indicators of female detection level will be higher or lower than the male.
【作者单位】：四川省中医药科学院;
【基金】：四川省科技厅基础业务专项课题(A-2013N-11)
【分类号】：R-33

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