TRPC1缺失对小鼠脑神经细胞生存的影响

发布时间：2018-06-11 15:02

本文选题：瞬时受体电位通道 + 神经元　；参考：《中国病理生理杂志》2016年04期

【摘要】：目的:研究瞬时受体电位通道1(TRPC1)缺失对小鼠海马神经元生存的影响。方法:选用6月龄TRPC1基因敲除小鼠及其对照小鼠,通过神经元特异性标志物Neu N免疫染色、尼氏染色及TUNEL染色检测TRPC1敲除小鼠海马CA1、CA3及齿状回(DG)神经细胞的变化情况。通过Western blot检测TRPC1基因敲除小鼠促凋亡因子C/EBP同源蛋白(C/EBP homologous protein,CHOP)及凋亡相关蛋白cleaved caspase-3的表达水平。结果:Neu N免疫荧光和尼氏染色发现,TRPC1基因敲除小鼠海马CA1、CA3及DG区神经细胞显著减少;TUNEL染色检测发现TRPC1基因敲除小鼠以上区域神经细胞凋亡显著增加;Western blot结果显示,与对照小鼠相比,TRPC1基因敲除小鼠海马组织CHOP及cleaved caspase-3的水平均显著升高。结论:TRPC1基因缺失可导致小鼠海马神经元数量显著减少,其机制可能与TRPC1缺失促进神经细胞凋亡有关。
[Abstract]:Aim: to study the effect of the deletion of transient receptor potential channel 1 (TRPC1) on the survival of hippocampal neurons in mice. Methods: 6-month-old TRPC1 knockout mice and their control mice were used to detect the changes of hippocampal CA1mCA3 and dentate gyrus (DGG) neurons in TRPC1 knockout mice by Neu N immunostaining, Nissl staining and Tunel staining. Western blot was used to detect the expression of apoptosis promoting factor C / EBP homologous protein and apoptosis-related protein (cleaved caspase-3) in TRPC1 knockout mice. Results the neuronal cells in the hippocampal CA1pCA3 and DG region of the TRPC1 knockout mice were significantly decreased by using the 10% Neu N immunofluorescence and Nissl staining. The neuronal apoptosis in the above regions of TRPC1 knockout mice was significantly increased by blot. The levels of chop and cleaved caspase-3 in hippocampal tissue of TRPC1 knockout mice were significantly higher than those of control mice. Conclusion the deletion of the 1: TRPC1 gene can significantly decrease the number of hippocampal neurons in mice, and the mechanism may be related to the promotion of neuronal apoptosis by TRPC1 deletion.
【作者单位】：暨南大学药学院;深圳市疾病预防控制中心深圳市现代毒理学重点实验室;
【基金】：广东省自然科学基金资助项目(No.2014A030313715) 深圳市科技研发基金基础研究项目(No.JCYJ20130329103949650)
【分类号】：R363

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