重组人红细胞生成素对新型支气管肺发育不良早产鼠模型血管内皮生长因子表达的影响

发布时间：2018-06-12 08:38

本文选题：支气管肺发育不良 + 红细胞生成素　；参考：《重庆医学》2017年29期

【摘要】：目的观察重组人红细胞生成素(rhEPO)对新型支气管肺发育不良(BPD)早产鼠模型肺组织血管内皮生长因子(VEGF)蛋白及mRNA表达的影响。方法60只定期受孕SD大鼠于孕第15天孕囊内注射脂多糖(LPS)或磷酸盐缓冲液(PBS),孕第21天剖宫娩出早产鼠,早产鼠于生后6h内分为5组:PBS+空气组、PBS+高氧组、LPS+高氧组、PBS+高氧+rhEPO组、LPS+高氧+rhEPO组。rhEPO(1 200IU/kg)干预组于高氧暴露6h后开始第1次背部皮下注射,隔天1次,共7次。分别在高氧暴露第1、7、14天处死早产鼠,观察各组生存率、体质量、肺质量并计算肺质量/体质量比值,观察肺组织病理变化,采用蛋白质印迹法(Western blot)和反转录PCR(RT-PCR)法检测肺组织VEGF蛋白及mRNA表达水平。结果与PBS+空气组比较,经高氧干预后早产鼠生存率降低、体质量减轻、肺质量增加,病理损伤加重,肺组织VEGF蛋白及mRNA表达水平降低,并且以LPS+高氧组更明显(P0.05),肺质量/体质量比值增加(P0.05);rhEPO干预后上述指标得到相应改善(P0.05)。结论 rhEPO可能通过参与调解VEGF相关通路,对BPD模型早产鼠起到一定的干预和治疗作用,提高了生存率。
[Abstract]:Objective to investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of vascular endothelial growth factor (VEGF) protein and mRNA in the lung tissue of a new type of bronchopulmonary dysplasia (BPD) model. Methods Sixty SD rats were injected intraperitoneally with lipopolysaccharide (LPS) or phosphate buffer solution (PBS) on the 15th day of gestation, and preterm rats were delivered to the uterus on the 21st day of gestation. Within 6 hours after birth, preterm rats were divided into 5 groups: 1: PBS air group, PBS hyperoxia group, LPS hyperoxia rhEPO group. RhEPO1 200 IUU / kg group. The rats in the intervention group were injected subcutaneously for the first time after 6 hours of hyperoxia exposure, once every other day, for a total of 7 times. The preterm rats were killed on the 14th day after hyperoxia exposure. The survival rate, body mass, lung mass and lung mass / body mass ratio were calculated, and the pathological changes of lung tissue were observed. The expression of VEGF protein and mRNA in lung tissue was detected by Western blot and reverse transcription PCR- RT-PCR- PCR. Results compared with PBS air group, the survival rate, body mass, lung mass, pathological injury and the expression of VEGF protein and mRNA in preterm rats decreased after hyperoxia intervention. Moreover, the lung mass / body weight ratio increased in LPS hyperoxia group, and the above indexes were improved after the intervention of rhEPO. Conclusion rhEPO may play a role in intervention and treatment of preterm rats with bpd by mediating VEGF related pathway, which can improve the survival rate.
【作者单位】：桂林医学院附属医院新生儿科;桂林医学院附属医院研究生科;桂林医学院附属医院教学管理部;
【基金】：广西高校科学技术研究基金资助项目(KY2015LX254) 广西自然科学基金资助项目(2014GXNSFAA118152)
【分类号】：R-332;R722.6

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