不同浓度结核菌建立兔脊柱结核模型的对比研究

发布时间：2018-06-12 09:19

本文选题：脊柱结核 + 动物模型　；参考：《中国矫形外科杂志》2017年05期

【摘要】：[目的]应用不同浓度结核杆菌构建兔脊柱结核模型,探索在造模中较适合的菌量。[方法]42只新西兰兔随机分为5组,实验组3组共30只,每组10只;对照组10只;空白组2只。实验组及对照组动物经弗氏完全佐剂致敏后,在L_6上终板下方钻孔,塞入明胶海绵。实验组分别注入0.1 ml浓度为10~6CFU/ml,10~7CFU/ml,10~8CFU/ml H37Rv标准菌;对照组注入0.9%的生理盐水0.1 ml;空白组不做任何处理。[结果]术后12周兔存活率:注射10~6 CFU/ml菌量兔100%、10~7CFU/ml菌量80%、10~8CFU/ml菌量30%、对照组100%、空白组100%。术后8周X线片阳性率分别为33.3%,62.5%,66.7%;CT及三维CT阳性率分别为44.4%,75%,100%。术后12周X线片阳性率分别为44.4%,75%,100%;CT及三维CT阳性率分别为44.4%,100%,100%。术后12周解剖见17只兔椎体发生骨质破坏,部分有干酪样坏死物质形成,未见有腰大肌脓肿形成;病理学检查:片状分布的干酪样坏死物质,骨小梁排列紊乱及轮廓不清,周围见上皮样细胞及多核巨细胞包绕,局部有部分死骨碎片形成,周围见大量炎细胞浸润;实验组细菌培养阳性率分别为22.2%,75%,66.7%。对照组及空白组未出现上述结核影像学及病理改变。[结论]不同浓度H37Rv标准菌构建兔脊柱结核模型存在较大差异,10~6CFU/ml成功率较低;10~8CFU/ml死亡率及并发症较高;10~7CFU/ml造模成功率高且死亡率较低,较适合兔脊柱结核模型的构建。
[Abstract]:[objective] to establish a rabbit spinal tuberculosis model with different concentrations of Mycobacterium tuberculosis, and to explore the appropriate amount of bacteria in the model. [methods] Forty-two New Zealand rabbits were randomly divided into 5 groups: experimental group (n = 30), control group (n = 10), control group (n = 10) and blank group (n = 2). The experimental and control groups were sensitized by Freund's complete adjuvant. The experimental group was injected with 0.1 ml 10 ~ 6 CFU / ml 10 ~ (7) CFU / ml 10 ~ (8) CFU / ml H37Rv standard bacteria, while the control group was given 0.9% normal saline 0.1 ml; the blank group was not treated with any treatment. [results] at 12 weeks after operation, the survival rate of rabbits was as follows: 10 ~ 6 CFU / ml, 100 ~ 107CFU / ml, 80 ~ 108CFU / ml, 100 / ml, 100%, 100%, 100%, 100%, 100%, 100 CFU / ml, 100% respectively. At 8 weeks after operation, the positive rates of X-ray films were 33.3 and 66.7and 66.7respectively. The positive rates of CT and 3D CT were 44.4and 75100, respectively. At 12 weeks after operation, the positive rates of X-ray films were 44.4 and 75 / 100, respectively. The positive rates of CT and 3D CT were 44.4 and 100%, respectively. At 12 weeks after operation, 17 rabbits were found to have bone destruction, some caseous necrotic substances were formed, and no abscess of psoas major muscle was found. Epithelioid cells and multinucleated giant cells were wrapped around, some dead bone fragments were formed locally, and a large number of inflammatory cells were infiltrated around them. The positive rates of bacterial culture in the experimental group were 22. 2% and 66. 7%, respectively. The imaging and pathological changes were not found in control group and blank group. [conclusion] the success rate of 106CFU / ml was lower than that of 108CFU / ml, and the mortality rate of 107CFU / ml was higher than that of 107CFU / ml, which was more suitable for the establishment of spinal tuberculosis model in rabbits with different concentrations of H37Rv standard bacteria.
【作者单位】：兰州军区兰州总医院骨科中心;
【基金】：国家自然科学基金资助项目(编号:81371983) 甘肃省自然科学基金资助项目(编号:1308RJZA146)
【分类号】：R529.2;R-332

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