年龄对猕猴骨髓间充质干细胞体外分化和细胞因子水平的影响

发布时间：2018-06-13 12:13

本文选题：骨髓 + 间质干细胞　；参考：《中国组织工程研究》2017年25期

【摘要】：背景:间充质干细胞因其良好的生物学特性和广泛的应用前景而受到关注,但随着个体衰老和体外培养时间的增加,细胞逐渐呈现衰老的特征。非人灵长类动物与人具有相似的生物学特性,在动物模型和疾病治疗研究方面具有独特的优势。目的:分析不同年龄段猕猴骨髓间充质干细胞的增殖和分化情况,探讨年龄对骨髓间充质干细胞的影响及可能机制。方法:通过骨髓穿刺术采集3岁以内和20岁以上雄性猕猴的骨髓,分为幼年组和老年组,每组3只,分离骨髓间充质干细胞进行体外培养,观察其形态变化、增殖能力和分化能力,进行衰老相关的β-半乳糖苷酶染色,ELISA法和蛋白芯片检测细胞因子水平。结果与结论:随着年龄的增长,老年猕猴骨髓间充质干细胞的增殖、分化能力显著降低,衰老细胞显著增加,细胞因子白细胞介素1b、白细胞介素4、白细胞介素6、肿瘤坏死因子α和血管内皮生长因子水平显著降低,肝素结合碱性成纤维细胞生长因子和胎盘生长因子水平显著升高,说明骨髓间充质干细胞的增殖、分化、旁分泌等功能会因衰老而逐渐受到损害。
[Abstract]:Background: mesenchymal stem cells (MSCs) have attracted much attention due to their good biological characteristics and wide application prospects. However, with the increase of individual senescence and culture time in vitro, the cells gradually present the characteristics of senescence. Nonhuman primates have similar biological characteristics to humans and have unique advantages in animal models and disease therapy. Aim: to analyze the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) in rhesus monkeys at different ages, and to explore the effect of age on bone marrow mesenchymal stem cells (BMSCs) and its possible mechanism. Methods: bone marrow of male rhesus monkeys aged less than 3 years old and over 20 years old were collected by bone marrow puncture and divided into juvenile group and senile group. Bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured in vitro, and their morphological changes were observed. The levels of cytokines were detected by 尾 -galactosidase staining Elisa and protein chip assay. Results and conclusion: the proliferation and differentiation ability of bone marrow mesenchymal stem cells in old rhesus macaques were significantly decreased and the aging cells were significantly increased with the increase of age. The levels of cytokines interleukin 1b, interleukin 4, interleukin 6, tumor necrosis factor 伪 and vascular endothelial growth factor decreased significantly, while heparin binding basic fibroblast growth factor and placental growth factor increased significantly. These results indicate that the proliferation, differentiation and paracrine function of bone marrow mesenchymal stem cells are gradually impaired by aging.
【作者单位】：贵州医科大学;解放军成都军区昆明总医院细胞生物治疗中心细胞生物医药技术国家地方联合工程实验室云南省细胞治疗技术转化医学重点实验室;解放军第三军医大学研究生管理大队;
【基金】：云南省创新团队(2015HC020) 昆明市重点项目(2015-1-S-00973) 全军实验动物专项(SYDW[2014]003)~~
【分类号】：R329.2

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