去铁蛋白双金簇可控自组装及其生物医学成像的研究

发布时间：2018-06-13 12:44

本文选题：金簇 + 去铁蛋白　；参考：《吉林大学》2011年硕士论文

【摘要】：有良好生物兼容性,低毒性,和稳定性的可以靶向组织或细胞纳米结构在纳米生物医学上已经引起了很大的兴趣。本文成功用“点控制”的技术在去铁蛋白内部可控合成两个协同作用的可以经激发产生荧光的金簇。并通过测定荧光光谱,证实随着金簇尺寸的增大和之间距离的减少,两金簇之间的能量共振转移(Forster resonance energy transfer, FRET)会使两个金簇总的荧光强度逐渐增强,并使荧光光谱发生红移。通过冷冻电镜(Cryo-electron microscopy)和透射电镜(transmission electron microscopy)的低分辨,高分辨模式和扫描模式分别得到成对金簇在蛋白内部的直观图像。在扫描模式下还得到金元素的X射线能量散射图谱(energy-dispersive-X-ray spectroscopy, EDX)证明确实是在去铁蛋白中组装成功双金簇。通过测定Au-Ft (apoferritin-far-red Au cluster)的X-射线近边吸收峰,证明是在去铁蛋白的两个重链上组装上的双金簇。成功的在有去铁蛋白受体的Caco-2细胞及小鼠的肾脏部位得到了特定的生物医学靶向荧光成像。这些实验结果表明,利用生物体本身存在的具有靶向功能的蛋白质和无机纳米材料组装体,有可能成为一种解决包括肿瘤在内的各种难以攻克疾病的诊断及治疗的潜在方法。相关文章已经被JACS (IF=8.58)接收。
[Abstract]:Good biocompatibility, low toxicity, and stability of targeted tissue or cell nanostructures have attracted considerable interest in nanomedicine. In this paper, the "point control" technique has been successfully used to control the synthesis of two clusters of gold which can excite and produce fluorescence within the deferritin. The fluorescence spectra show that with the increase of the gold cluster size and the decrease of the distance between the two gold clusters, the energy resonance transfer resonance energy transfer, fret between the two gold clusters will gradually increase the total fluorescence intensity of the two gold clusters, and make the fluorescence spectrum red shift. The low resolution, high resolution and scanning modes of cryopreservation electron microscopy (Cryo-electron microscopy) and transmission electron microscopy (electron) were used to obtain the visual images of gold clusters inside the protein, respectively. The X-ray energy scattering spectra of gold elements were also obtained in scanning mode. It was proved that the double gold clusters were successfully assembled in ferritin. By measuring the X- ray absorption peak of Au-Ft apoferritin-far-red gold cluster, it is proved that it is a double gold cluster assembled on two heavy chains of ferritin. Specific biomedical targeted fluorescence imaging was successfully obtained in Caco-2 cells with ferritin receptor and in the kidney of mice. These results indicate that the use of proteins and inorganic nanomaterials with targeted function, which exists in organisms themselves, may become a potential method for the diagnosis and treatment of various difficult diseases, including tumors. Related articles have been received by JACS IFF 8.58).
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R341

【共引文献】