IL-6、TNF-α在术后肠梗阻动物模型中的表达及中药腑安的保护作用研究
发布时间：2018-06-13 16:51
本文选题：术后肠梗阻 + 腑安冲剂 ； 参考：《大连医科大学》2011年硕士论文
【摘要】：目的:建立术后肠梗阻动物模型,探讨腹部手术后血清IL-6、TNF-α表达水平与术后肠梗阻的关系及中药腑安的保护作用机制。 方法:KM小鼠45只,体重20±2g,随机分为3组,即腑安组、生理盐水组及空白对照组,每组15只。腑安组及生理盐水组小鼠均接受开腹手术以建立术后肠梗阻模型,术后2h两组分别给予腑安药液0.3ml及生理盐水0.3ml灌胃。空白对照组未行手术。采用酚红定量法及酚红排空法测定术后24h胃排空率及小肠推进率,处死前摘眼球取血1ml测定血清IL-6及TNF-α水平。应用SPSS17.0统计软件进行数据分析,以P0.05为显著性标准。各组间计量资料比较采用方差分析,两变量相关性分析采用线性相关并绘制散点图。 结果: 1、空白对照组术后24h胃排空率(%)为49.33±6.22;生理盐水组术后24h胃排空率(%)为26.25±2.73;腑安组术后24h胃排空率(%)为44.76±4.07;生理盐水组与空白对照组相比术后24h胃排空率显著降低(P0.05);腑安组与生理盐水组相比术后24h胃排空率显著改善(P0.05)。 2、空白对照组术后24h小肠推进率(%)为50.82±5.07;生理盐水组术后24h小肠推进率(%)为33.86±3.13;腑安组术后24h小肠推进率(%)为48.48±5.12;生理盐水组与空白对照组相比术后24h小肠推进率显著降低(P0.05);腑安组与生理盐水组相比术后24h小肠推进率显著改善(P0.05)。 3、空白对照组血清IL-6浓度(pg/ml)为103.29±14.16;生理盐水组血清IL-6浓度(pg/ml)为202.41±17.08;腑安组血清IL-6浓度(pg/ml)为126.59±19.64;生理盐水组血清IL-6水平显著高于空白对照组(P0.05);腑安组血清IL-6水平显著低于生理盐水组(P0.05)。 4、空白对照组血清TNF-α浓度(pg/ml)为27.58±11.05;生理盐水组血清TNF-α浓度(pg/ml)为61.43±15.23;腑安组血清TNF-α浓度(pg/ml)为35.52±11.31;生理盐水组血清TNF-α水平明显高于空白对照组(P0.05);腑安组血清TNF-α水平显著低于生理盐水组(P0.05)。 5、术后24h胃排空率与血清IL-6呈负相关,r=㧟0.948;术后24h小肠推进率与血清IL-6呈负相关,r=㧟0.844;术后24h胃排空率与血清TNF-α呈负相关,r=㧟0.877;术后24h小肠推进率与血清TNF-α呈负相关,r=㧟0.779。 结论: 1、生理盐水组与空白对照组相比术后24h胃排空率及术后24h小肠推进率明显降低;腑安组与生理盐水组相比术后24h胃排空率及术后24h小肠推进率显著改善。这表明手术操作对胃肠动力具有明显抑制作用,腑安冲剂能够显著增加小鼠的术后24h胃排空率及小肠推进率,对于术后胃肠动力具有明显的改善作用。 2、生理盐水组血清IL-6及TNF-α水平明显高于空白对照组;腑安组血清IL-6及TNF-α水平显著低于生理盐水组。这表明手术操作能够导致炎症介质的释放,腑安冲剂能够抑制炎症介质的释放,对术后肠道炎症具有明显的抑制作用。 3、术后24h胃排空率与血清IL-6呈负相关,r=㧟0.948;术后24h小肠推进率与血清IL-6呈负相关,r=㧟0.844;术后24h胃排空率与血清TNF-α呈负相关,r=㧟0.877;术后24h小肠推进率与血清TNF-α呈负相关,r=㧟0.779。这表明术后胃肠动力的抑制与血清IL-6及TNF-α水平升高密切相关,胃肠的炎症反应程度与胃肠动力呈明显负相关。
[Abstract]:Objective: to establish an animal model of postoperative intestinal obstruction and to explore the relationship between the expression of serum IL-6, TNF- alpha and intestinal obstruction after abdominal operation and the protective mechanism of the traditional Chinese medicine.
Methods: 45 KM mice, with a weight of 20 + 2G, were randomly divided into 3 groups, namely, Fu An Group, physiological saline group and blank control group, 15 rats in each group. The abdominal operation was performed to establish the intestinal obstruction model in the Fu An Group and the normal saline group. After the operation, the 2H two groups were given the 0.3ml and the physiological saline 0.3ml respectively. The blank control group was not operated. The rate of 24h gastric emptying and the propulsive rate of small intestine were measured by phenol red quantitative method and phenol red emptying method. The levels of serum IL-6 and TNF- alpha were measured by 1ml before death. The data were analyzed with SPSS17.0 statistical software and P0.05 was a significant standard. The measurement data of each group were compared with variance analysis, and the correlation analysis of two variables was linear correlation. And draw the scatter plot.
Result:
1, the gastric emptying rate (%) of 24h in the blank control group was 49.33 + 6.22, and the 24h gastric emptying rate (%) was 26.25 + 2.73 after operation in the normal saline group, and the 24h gastric emptying rate (%) was 44.76 + 4.07 after the operation in the Fu An group. The 24h gastric emptying rate in the physiological saline group and the blank control group were significantly lower than that in the blank control group (P0.05), and the 24h gastric emptying rate was significant compared with the normal saline group after operation. Improvement (P0.05).
2, the 24h small intestinal propulsive rate (%) in the blank control group was 50.82 + 5.07, and the 24h small intestinal propulsion rate (%) was 33.86 + 3.13 after operation in the normal saline group, and the 24h small intestinal propulsive rate (%) was 48.48 + 5.12 in the Fu group after operation, and the 24h small intestinal propulsive rate in the physiological saline group compared with the blank control group was significantly lower than that in the blank control group (P0.05), and the 24h small intestine was compared with the normal saline group after operation compared with the saline group. The propelling rate improved significantly (P0.05).
3, the serum concentration of IL-6 (pg/ml) in the blank control group was 103.29 + 14.16, the serum IL-6 concentration (pg/ml) in the normal saline group was 202.41 + 17.08, the serum IL-6 concentration (pg/ml) was 126.59 + 19.64 in the Fu An Group, and the serum IL-6 level in the saline group was significantly higher than that in the blank control group (P0.05), and the serum IL-6 level was significantly lower than that of the normal saline group (P0.05).
4, the serum TNF- alpha concentration (pg/ml) in the blank control group was 27.58 + 11.05, the serum TNF- alpha concentration (pg/ml) in the saline group was 61.43 + 15.23, the serum TNF- alpha concentration (pg/ml) was 35.52 + 11.31 in the Fu An Group, and the serum level of TNF- a in the saline group was significantly higher than that in the blank control group (P0.05), and the serum TNF- alpha level was significantly lower than that of the normal saline group (P0.05).
5, 24h gastric emptying rate was negatively correlated with serum IL-6, r=? 0.948, and 24h small intestinal propulsion rate was negatively correlated with serum IL-6, r=? 0.844; 24h gastric emptying rate was negatively correlated with serum TNF- a after operation, r=? 0.877; 24h small intestine propelling rate was negatively correlated with TNF- alpha in serum after operation, r=? R=?
Conclusion:
1, compared with the blank control group, the gastric emptying rate of 24h and the 24h intestinal propulsive rate after operation were significantly lower than those in the blank control group, and the gastric emptying rate and the 24h small intestinal propulsion rate after operation were significantly improved after the operation in the Fu An Group and the normal saline group. This showed that the operation has obvious inhibitory effect on the gastrointestinal motility, and the Fu Fu an granule can significantly increase the operation of the mice. Post 24h gastric emptying rate and intestinal propulsion rate significantly improved gastrointestinal motility after operation.
2, the level of serum IL-6 and TNF- a in the normal saline group was significantly higher than that in the blank control group; the level of serum IL-6 and TNF- a in the Fu An group was significantly lower than that in the normal saline group. This indicated that the operation could lead to the release of the inflammatory mediators, and the Fu an granule could inhibit the release of the inflammatory mediators, and had an obvious inhibitory effect on the intestinal inflammation after the operation.
3, 24h gastric emptying rate was negatively correlated with serum IL-6, r=? 0.948, and 24h small intestinal propulsion rate was negatively correlated with serum IL-6, r=? 0.844; 24h gastric emptying rate was negatively correlated with serum TNF- a after operation, r=? 0.877; 24h small intestinal propulsion rate was negatively correlated with serum TNF- alpha after operation; r=? The level of gastrointestinal inflammation was significantly negatively correlated with gastrointestinal motility.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R285.5
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