利用表达谱芯片数据筛选不同表达丰度的内参基因

发布时间：2018-06-13 17:48

本文选题：内参基因 + 表达谱芯片　；参考：《中国实验动物学报》2017年01期

【摘要】：目的基于表达谱芯片的检测结果,筛选多个内参基因,用于小家鼠肝脏组织中具有不同表达丰度基因的定量检测。方法应用表达谱芯片技术,完成小鼠肝脏组织的表达谱检测;依据基因表达丰度将基因分为3组,并进一步通过变异系数(coefficient of variation,CV)组内筛选候选内参基因;采用实时荧光定量PCR技术(realtime quantitative polymerase chain reaction,q PCR)和ge Norm软件确定内参基因。结果表达谱芯片成功采集超过60000个小鼠肝脏组织中转录本的表达量数据,并将之分为低、中、高3个组合。最终筛选了低表达Casp2和Lrrc14、中表达Nrd1和Trpc4ap、高表达Atp5a1和Clu,共6个内参基因。结论基于表达谱芯片数据筛选的6个内参基因,可适用于q PCR技术准确定量小家鼠肝组织转录组中不同表达丰度基因的表达量。
[Abstract]:Objective to screen multiple internal reference genes based on the results of expression microarray for quantitative detection of differentially expressed abundant genes in rat liver. Methods the expression profiles of mouse liver tissues were detected by using expression microarray technique, and the genes were divided into 3 groups according to the abundance of gene expression, and the candidate internal reference genes were screened by coefficient of variation (CV) group. Real time quantitative polymerase chain reactionQ PCR and ge Norm software were used to identify the internal reference gene. Results the expression data of the transcripts in more than 60000 mouse liver tissues were successfully collected by expression microarray and divided into three combinations: low, medium and high. Finally, the low expression of Casp2 and Lrrc14, the expression of Nrd1 and Trpc4apand the high expression of Atp5a1 and Club were screened. Conclusion the six internal reference genes screened based on the expression microarray data can be used to accurately quantify the expression of different abundance genes in rat liver tissue transcriptiongroup by qPCR.
【作者单位】：东华大学生物科学与技术研究所;
【基金】：国家自然科学基金面上项目(编号:31371257) 上海市科委关键项目(编号:12140900404,13140900300,15140900500)
【分类号】：R-332

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