急性痛风性关节炎大鼠模型的建立及模型维持时间观察

发布时间：2018-06-14 10:37

  本文选题：急性痛风性关节炎 + 大鼠模型　； 参考：《中国实验动物学报》2017年05期

【摘要】：目的建立急性痛风性关节炎(acute gouty arthritis,AGA)大鼠模型并观察其维持时间。方法采用25 mg/m L尿酸钠(monosodium urate,MSU)晶体混悬液踝关节腔注射复制大鼠AGA模型,多个时间点动态观察8 d,以大鼠受试踝关节局部皮温、肿胀度、步态、关节液炎性细胞及其滑膜组织病理形态学改变等指标判断是否成模及其维持时间。结果造模后3 h,生理盐水组和模型组均可见踝关节肿胀,皮温升高,步态异常,关节液炎性细胞数增多,滑膜组织增生、毛细血管充血、滑膜细胞排列紊乱等炎症表现,两组以上指标与空白组比较差异均有显著性(P0.01);造模后4 h,生理盐水组以上炎症表现明显减轻,较3 h时差异有显著性(P0.01),而模型组较3h时加重(P0.01),并且与生理盐水组比较差异有显著性(P0.01);造模后24 h,生理盐水组各项指标恢复正常,而模型组炎症继续加重;造模后48~72 h,模型组肿胀、皮温、步态异常等局部炎症达到高峰;造模后96~168h,模型组踝关节局部炎症逐渐减轻,但各项指标与空白组比较差异仍有显著性(P0.01);造模后192 h,模型组肿胀、皮温、步态异常等外在炎症表现恢复正常,而炎性细胞数及滑膜病理变化与空白组比较差异仍均有显著性(P0.01)。结论采用MSU晶体混悬液踝关节腔注射可在造模后4 h成功制备并鉴定出AGA大鼠模型,且至少能维持到造模后168 h。
[Abstract]:Objective to establish the model of acute gouty arthritis (AGA) rats with acute gouty arthritis and observe its maintenance time. Methods the rat model was made by injection of 25 mg/m L sodium uric acid sodium monosodium urateate (MSU) crystal suspension into the ankle joint. Dynamic observation was made at multiple time points for 8 days. The local skin temperature, swelling degree and gait of the ankle joint were observed at different time points. The pathological and morphological changes of synovium and inflammatory cells in articular fluid were used to determine whether the model was formed and the time of maintenance. Results the swelling of ankle joint, elevation of skin temperature, abnormal gait, increased number of inflammatory cells in synovial fluid, proliferation of synovial tissue, capillary hyperemia, disorder of synovial cell arrangement and other inflammatory manifestations were observed in saline group and model group 3 hours after modeling. The above indexes in the two groups were significantly different from those in the blank group (P 0.01), and the inflammatory manifestations of the above groups in the saline group were significantly reduced at 4 h after the establishment of the model. There was a significant difference in P0.01D between the model group and the saline group at 3 h, and the difference was significant in the model group compared with the saline group at 3 h, the indexes of the saline group returned to normal at 24 h after modeling, but the inflammation in the model group continued to increase. The swelling, skin temperature and gait abnormality reached the peak in the model group at 48h 72 h after modeling, and the local inflammation of the ankle joint decreased gradually at 96h 168h after modeling, but there were significant differences between the indexes of the model group and the blank group (P 0.01), the swelling of the model group at 192 h after modeling. The number of inflammatory cells and the pathological changes of synovial membrane were significantly different from those of the blank group (P 0.01). Conclusion the rat model of AGA can be successfully prepared and identified by injection of MSU crystal suspension into ankle joint at 4 hours after the establishment of the model, and can be maintained at least 168 hours after the establishment of the model.
【作者单位】： 福建中医药大学;福建卫生职业技术学院;
【基金】：国家自然科学基金资助项目(No.81473495)
【分类号】：R-332;R589.7
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本文编号：2017095