一种水溶性免疫佐剂应用于小分子化合物单克隆抗体制备的效果评价（英文）

发布时间：2018-06-14 14:46

本文选题：水溶性免疫佐剂 + 单克隆抗体　；参考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2016年04期

【摘要】：目的:评价一种新型水溶性免疫佐剂在制备小分子化合物单克隆抗体(单抗)中的应用效果。创新点:采用一种新型水溶性免疫佐剂,快速制备了分别针对4种农药和1种海洋毒素的高亲合力单抗,且其性能与以往采用弗氏佐剂获得的相应单抗非常接近。方法:本研究以4种农药和1种海洋毒素为目标分析物,采用一种新型水溶性佐剂与小分子抗原相互混合,直接对Balb/c小鼠进行小腿肌肉注射免疫。经过2~3次免疫后进行腹腔注射常规末次免疫,细胞融合后筛选出了相应的杂交瘤细胞株,制备并鉴定了针对上述半抗原小分子的特异性单抗。结论:采用该水溶性免疫佐剂制备抗原注射溶液无需乳化过程,免疫操作简便,且对小鼠负面效应较小;虽然该处理小鼠产生的抗血清效价并没有像常规弗氏免疫佐剂带来的效价值高,但细胞融合后筛选得到有效细胞株的概率尚可;获得的针对目标分析物的单抗在灵敏度和特异性等方面都接近于以往采用弗氏佐剂获得的单抗。试验结果表明,该水溶性免疫佐剂适用于针对小分子化合物单抗的高效制备。
[Abstract]:Aim: to evaluate the application of a novel water-soluble immune adjuvant in the preparation of monoclonal antibodies (McAbs) against small molecular compounds. Innovation: a novel water-soluble immunoadjuvant was used to prepare high affinity McAbs for four pesticides and one marine toxin, respectively, and their properties were very similar to those obtained by Freund's adjuvant. Methods: in this study, four pesticides and one marine toxin were used as target analytes. Balb / c mice were immunized with a new water-soluble adjuvant and small molecular antigen directly by intramuscular injection. The hybridoma cell lines were screened after intraperitoneal injection of routine final immunization after 23 immunizations and the specific monoclonal antibodies against these hapten small molecules were prepared and identified. Conclusion: using the water-soluble immune adjuvant to prepare the antigen injection solution does not need emulsifying process, the immunological operation is simple, and the negative effect on mice is small. Although the titer of antiserum produced by this treatment was not as high as that of conventional Freund's immune adjuvant, the probability of cell fusion screening for effective cell line was still acceptable. The sensitivity and specificity of the McAbs obtained were similar to those obtained by Freund's adjuvant. The experimental results show that the water-soluble immune adjuvant is suitable for the preparation of monoclonal antibodies against small molecular compounds.
【作者单位】： Institute
【基金】：supported by the Special Fund for Agro-scientific Research in the Public Interest(No.201203094-3) the Scientific Research Fund of Zhejiang Provincial Education Department(No.Y201329970) the National Natural Science Foundation of China(No.31401768) the Experimental Technology Research Project of Zhejiang University(No.SZD201404),China
【分类号】：R392-33

【相似文献】