白花蛇舌草对Renca肾癌细胞模型小鼠凋亡相关蛋白Fas、caspase3及caspase7表达影响

发布时间：2018-06-14 18:07

本文选题：白花蛇舌草 + 肾癌　；参考：《中国生化药物杂志》2016年12期

【摘要】：目的探讨中药白花蛇舌草对Renca肾癌细胞模型小鼠凋亡相关蛋白Fas、caspase3及caspase7表达的影响。方法选取BALB/C小鼠120只,随机分为正常对照组、模型对照组、白细胞介素组、白花蛇舌草组,各30只,雌雄各半。除正常对照组外,各组均建立肾癌模型,并给予相应的药物治疗。观察所有小鼠的肿瘤体积及重量、抑瘤率,对Fas、Fas L、caspase3及caspase7蛋白表达水平进行检测。结果与模型对照组比较,白花蛇舌草组、白细胞介素组小鼠的肿瘤体积、瘤体重量较低,抑瘤率较高,Fas阳性表达率及Fas蛋白表达较高,Fas L阳性表达率及Fas L蛋白表达较低,caspase3、caspase7阳性表达率及蛋白表达水平较高,差异均有统计学意义(P0.05)。其中白花蛇舌草组以上指标均优于白细胞介素组(P0.05)。结论白花蛇舌草能够明显促进肾癌模型小鼠凋亡相关蛋白Fas、caspase3及caspase7表达,抑制Fas L蛋白表达,提高抑瘤率。
[Abstract]:Objective to investigate the effect of Herba Phellodendron on the expression of apoptosis-related protein Fas-caspase3 and caspase7 in Renca renal cancer cell model mice. Methods 120 BALB / C mice were randomly divided into three groups: normal control group, model control group, interleukin group, 30 mice in each group. In addition to the normal control group, each group established renal cell carcinoma model, and given the corresponding drug treatment. The tumor volume and weight, tumor inhibition rate and the expression of FAS FAS caspase3 and caspase7 protein in all mice were observed. Results compared with the model control group, the tumor volume and tumor weight of mice in the group of Hedyotis albicans and the group of interleukin were lower than those in the control group. The tumor inhibition rate was higher, the positive rate of FAS protein was higher than that of FAS protein, and the positive rate of FAS L protein and the expression of FAS L protein were lower. The positive rate and protein expression level of caspase3 and caspase7 were higher than those of the control group, and the difference was statistically significant (P 0.05). The above indexes were better than that of interleukin group (P 0.05). Conclusion Apoptosis-associated protein Fas-caspase3 and caspase7 in renal cell carcinoma model mice can be increased, FAS L protein expression is inhibited and tumor inhibition rate is increased.
【作者单位】：天津市南开医院泌尿外科;
【分类号】：R285.5;R-332

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