旋毛虫与Hela细胞相关抗原抗体抗肿瘤作用的研究

发布时间：2018-06-16 13:53

本文选题：旋毛虫 + Hela细胞　；参考：《吉林大学》2011年硕士论文

【摘要】：为了深入研究旋毛虫抗肿瘤的的作用,本试验先在体外利用MTT和细胞凋亡的检测的方法初步确定了旋毛虫可溶性抗原可以使Hela细胞发生凋亡,在用ELISA方法初步确定了旋毛虫与Hela细胞间存在共同抗原,并利用SDS-PAGE和Western-blotting方法测得了相关抗原的分子量。运用离心超滤装置及透析等技术对相关抗原进行了分离,FITC-免疫组化法对该抗原进行了定位分析。在此基础上,建立了Hela细胞裸鼠移植瘤动物模型,并对旋毛虫与Hela细胞相关抗原抗体进行了抗肿瘤实验。实验结果显示:通过MTT法和细胞凋亡实验证明,旋毛虫可溶性抗原可以对Hela细胞的增殖具有抑制作用,ELISA法初步证明旋毛虫与Hela细胞间存在较强的免疫交叉反应;利用SDS-PAGE和Western-blotting方法获得了旋毛虫抗原与Hela细胞相关抗原的分子量分别是40KDa和55KDa。免疫组化定位分析该相关抗原位于Hela细胞表面抗原和旋毛虫的消化道上皮细胞膜上;应用相关抗原抗体在裸鼠体内进行抗肿瘤试验表明,相关抗原抗体具有抗肿瘤作用,抑瘤率为82.3%,且效果略好于可溶性抗原抗体组。首次以旋毛虫与Hela细胞相关抗原抗体为切入点研究旋毛虫抗肿瘤的机理,为旋毛虫抗肿瘤机制的研究与开发提供了理论依据。
[Abstract]:In order to study the antitumor effect of Trichinella spiralis in vitro, MTT and apoptosis were used to determine that soluble antigen of Trichinella spiralis could induce apoptosis in Hela cells. The common antigen between Trichinella spiralis and Hela cells was preliminarily determined by Elisa, and the molecular weight of the related antigen was measured by SDS-PAGE and Western-blotting. The antigens were isolated by centrifugation ultrafiltration and dialysis. The antigen was localized by FITC- immunohistochemical method. On this basis, an animal model of Hela cell xenografts in nude mice was established, and the antitumor effects of Trichinella spiralis and Hela cell related antigens were tested. The results showed that the soluble antigen of Trichinella spiralis could inhibit the proliferation of Hela cells by MTT assay and apoptosis assay. Elisa showed that there was a strong immune cross reaction between Trichinella spiralis and Hela cells. The molecular weights of Trichinella spiralis antigen and Hela cell associated antigen were 40 KDa and 55 KDa respectively by SDS-PAGE and Western-blotting. Immunohistochemical analysis showed that the associated antigen was located on the surface antigen of Hela cell and the membrane of digestive tract epithelium of Trichinella spiralis. The inhibition rate was 82.3%, and the effect was slightly better than that of soluble antigen antibody group. The antitumor mechanism of Trichinella spiralis was studied by using the antibody against Hela cells for the first time, which provided a theoretical basis for the research and development of antitumor mechanism of Trichinella spiralis.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R392;R73-35

【引证文献】