超顺磁氧化铁标记对干细胞转铁蛋白受体和铁蛋白轻链基因及蛋白表达的影响

发布时间：2018-06-17 01:45

本文选题：超顺磁氧化铁 + 脂肪干细胞　；参考：《生物医学工程与临床》2013年01期

【摘要】：目的研究超顺磁氧化铁(SPIO)标记对大鼠脂肪干细胞(ADSCs)转铁蛋白受体(TfR)和铁蛋白轻链(Fn-L)基因及蛋白表达的影响。方法实验通过医院伦理委员会的批准。标记组(实验组)采用多聚赖氨酸(PLL,终质量浓度为1.5μg/mL)介导SPIO(终质量浓度为50μg/mL)标记ADSCs。在2、4、8、16、24、96、168、336、504、672 h,分别用实时荧光定量聚合酶链反应(RT-PCR)和WesternBlot实验定量检测实验组和未标记组(对照组)TfR和Fn-L基因及蛋白表达水平。结果 PLL-SPIO标记ADSCs后,实验组Fn-L mRNA(2、4、8、16、24、96、168、336 h)及蛋白(16、72、96、168 h)(P均0.05)表达水平会暂时性升高,至标记后一定时间,Fn-L mRNA(504、672h)及蛋白(336、504、672h)表达水平两组间差异无统计学意义(P均0.05);实验组TfR mRNA(2、4、8、16、72、96、168、336 h)及蛋白(24、96 h)(P均0.05)表达水平会暂时性减低,至标记后一定时间,TfRmRNA(504、672 h)及蛋白(168、336、504、672 h)表达水平两组间差异无统计学意义(P均0.05)。结论在一定浓度内,PLL-SPIO标记ADSCs,对Fn-L和TfR基因及蛋白表达仅产生暂时性影响;从而为细胞内标记过程的安全性提供了实验依据。
[Abstract]:Objective to study the effects of superparamagnetic iron oxide (SPIOO) labeling on the expression of transferrin receptor (TfR) and ferritin light chain (Fn-L) gene and protein in rat adipose stem cell (ADSCs). Methods the experiment was approved by the hospital ethics committee. The labeled group (experimental group) was labeled with Polysylysine (PLL) at a final concentration of 1.5 渭 g / mL (50 渭 g / mL). The expression levels of TfR and Fn-L genes and proteins in experimental and unlabeled groups were quantitatively detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and Western Blot (control group). Results after PLL-SPIO-labeled ADSCs, the expression levels of Fn-L mRNA2, Fn-L mRNA2, 2, 4, 6, 96, 16, 56, 336 (h) and P0. 05) were temporarily increased in the experimental group, respectively. There was no significant difference in the expression level of Fn-L mRNA-504672h and protein 336504672h between the two groups (P < 0.05); the expression levels of TfR mRNA-2FR-mRNA-1672c96168336 h) and protein 2496hP0.05) were temporarily decreased in the experimental group. There was no significant difference between the two groups in the expression level of TfRmRNA-50467hand protein 16833650467h. there was no significant difference between the two groups (P < 0.05). Conclusion the expression of Fn-L and TfR genes and proteins in ADSCslabeled with PLL-SPIO at a certain concentration has only a temporary effect, which provides an experimental basis for the safety of the intracellular labeling process.
【作者单位】：广东省医学科学院广东省人民医院放射科;
【基金】：国家自然科学基金资助项目(30970798)
【分类号】：R329

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