纳米氧化铈、氧化镧和氧化钇对原代培养的小鼠成骨细胞增殖、分化及矿化功能的影响

发布时间：2018-06-17 21:04

本文选题：稀土纳米材料 + 成骨细胞　；参考：《河北大学》2012年硕士论文

【摘要】：本文以纳米CeO_2、La_2O_3和Y_2O_3三种稀土纳米材料为研究对象，在对其理化性质充分表征的基础上，以原代分离的小鼠成骨细胞为模型，采用四甲基偶氮唑蓝法、碱性磷酸酶活性检测、油红O染色、茜素红染色等测定方法在细胞水平研究了纳米CeO_2、La_2O_3和Y_2O_3对成骨细胞增殖、分化及矿化功能的影响；并采用流式细胞仪对CeO_2纳米颗粒的摄取情况进行了研究。结果表明： 1.实验所用的纳米材料分别为：粒径分别约为30nm和50nm的近似为球形的CeO_2纳米颗粒，形貌分别为不规则片状和短棒状的纳米La_2O_3，粒径约为30nm的近似为球形纳米Y_2O_3，这几种纳米材料在培养基中均发生了不同程度的团聚现象。 2.纳米CeO_2对小鼠原代成骨细胞增殖、分化及矿化功能的影响依赖于纳米颗粒的尺寸、浓度和孵育时间等因素。50nm CeO_2对OBs的细胞增殖有一定抑制作用，但是30nm CeO_2在同样的剂量下则没有表现出抑制效果。两种尺寸的CeO_2纳米颗粒均能促进OBs矿化结节的形成，并且这种促进作用呈剂量依赖效应，但是促进效果随着纳米CeO_2的尺度减小而有增大的趋势。 3.纳米La_2O_3和Y_2O_3对小鼠原代成骨细胞增殖、矿化及成脂分化的影响依赖于纳米颗粒的形貌、剂量、化学组成和孵育时间等因素。同等剂量下片状纳米La_2O_3对细胞增殖的抑制效果明显高于棒状纳米La_2O_3的抑制作用，，纳米Y_2O_3的细胞毒性要低于同等剂量纳米La_2O_3；两种形貌的纳米La_2O_3在浓度0.6μg/mL以上时均能促进OBs矿化结节的形成，且促进作用呈剂量依赖效应，但是片状纳米La_2O_3对OBs矿化作用的促进率明显高于棒状纳米La_2O_3。 4. CeO_2纳米颗粒与成骨细胞作用24h后会进入到细胞内部，并且随浓度的增大进入细胞的量增多。
[Abstract]:In this paper, three kinds of rare earth nanomaterials, namely, CeO2L _ 2O _ 3 and Ys _ 2O _ 3, were used as research objects. On the basis of fully characterizing their physical and chemical properties, the primary isolated mouse osteoblasts were used as model, the activity of alkaline phosphatase was determined by tetramethyl azolyl blue method. Oil red O staining and alizarin red staining were used to study the effects of CeO2LAS and Y _ 2O _ 3 on the proliferation, differentiation and mineralization of osteoblasts at the cell level, and the uptake of CEO _ 2 nanoparticles by flow cytometry. The results show that: 1. The nanomaterials used in the experiment are: CeO-2 nanoparticles with approximately 30nm and 50nm particle sizes, which are approximately spherical, The morphology of La _ 2O _ 3 nanocrystalline is irregular flake and short rod shape, and the particle size of 30nm is approximately spherical Y _ 2O _ 3. These nanomaterials are agglomerated to different degrees in the medium. 2. The effects of CeO2 nanoparticles on the proliferation, differentiation and mineralization of primary mouse osteoblasts depended on the size, concentration and incubation time of nanoparticles. 50 nm CeO2 could inhibit the proliferation of osteoblasts. But 30nm CEO _ 2 showed no inhibitory effect at the same dose. Both CeO-2 nanoparticles could promote the formation of mineralized nodules of OBs, and the effect was dose-dependent, but the effect increased with the decrease of CeO2 size. 3. The effects of the nano La _ 2O _ 3 and Y _ S _ 2O _ 3 on the proliferation, mineralization and adipogenic differentiation of primary osteoblasts depend on the morphology, dosage, chemical composition and incubation time of the nanoparticles. At the same dose, the inhibitory effect of tippal-like nano-La2O3 on cell proliferation was significantly higher than that of rod-shaped nano-La2O3, and the cytotoxicity of nano-sized La2O3 was lower than that of the same dose of nano-La2O3; Both kinds of nano-La _ 2O _ 3 could promote the formation of mineralized nodules of OBs at concentrations above 0.6 渭 g / mL, and the effect was dose-dependent. However, the promotion rate of nano-La _ 2O _ 3 was significantly higher than that of rod-shaped nano-La2O3.4. After contact with osteoblasts for 24 hours, CeO-2 nanoparticles will enter into the cells and the amount of them will increase with the increase of concentration.
【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R329

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