不同浓度的大豆蛋白对高尿酸血症大鼠尿酸及肾功能的影响

发布时间：2018-06-18 01:50

本文选题：氧嗪酸钾 + 酵母粉　；参考：《暨南大学》2011年硕士论文

【摘要】：目的研究不同种类造模剂诱导高尿酸血症大鼠模型的可行性,以及食物中不同浓度的大豆蛋白对高尿酸血症动物模型尿酸代谢及肾功能的影响。方法第一部分：SD雄性大鼠20只,随机分为四组,分别予5%氧嗪酸钾、10%酵母粉、10%酵母粉+2%氧嗪酸钾和普通饲料饲喂3周,再继续给予普通饲料饲喂1周,每周测量大鼠体重,留取血、尿标本检测尿酸、肌酐、尿素氮、血脂、白蛋白。第二部分：20只高尿酸血症模型大鼠,随机分为4组,分别予11%大豆蛋白、22%大豆蛋白、44%大豆蛋白和22%大豆蛋白加用别嘌呤醇喂养6周,每周测量大鼠体重,留取血、尿标本检测尿酸、肌酐、尿素氮、血脂、白蛋白。结果第一部分： 1.与对照组相比,5%氧嗪酸钾组大鼠血尿酸在第1、2、3周均升高(P0.05)；10%酵母粉组血尿酸仅在第2周升高(P0.05)；10%酵母粉+2%氧嗪酸钾组血尿酸在第1、2周升高(P0.05),但在第3周下降,与对照组相比无统计学差异。停用造模剂1周后,三个造模组大鼠血尿酸水平与对照组相比均无差异。 2.造模组大鼠和对照组大鼠的血肌酐、尿素氮、白蛋白、体重、尿尿酸均无统计学差异。第二部分： 1.与普通大豆蛋白组相比,低大豆蛋白组和普通大豆蛋白加别嘌呤醇组大鼠在第4、5、6周血尿酸降低(P0.05),且血尿酸在低大豆蛋白组和普通大豆蛋白加别嘌呤醇组间无统计学差异；高大豆蛋白组大鼠在第2、3、4、5周血尿酸水平增高(P0.05)。 2.与普通大豆蛋白组相比,低大豆蛋白组大鼠血肌酐在第6周降低(P0.05),尿素氮在第2、3、4、6周降低(P0.05)；高大豆蛋白组大鼠的血肌酐、尿素氮水平均在第2、3周增高(P0.05)。 3.与普通大豆蛋白组相比,高大豆蛋白组大鼠的血清甘油三酯在第2、3周增高(P0.05)。 4.各组大鼠体重、白蛋白、血清胆固醇、尿尿酸、尿肌酐和尿蛋白排泄量无统计学差异。结论第一部分： 1.持续饲喂5%氧嗪酸钾可诱导稳定的高尿酸血症状态,可作为今后高尿酸血症的研究模型。 2. 5%氧嗪酸钾对实验大鼠的营养状况和肾功能均无影响。第二部分： 1.低大豆蛋白降低高尿酸血症大鼠模型的血尿酸,其降尿酸作用等同于别嘌呤醇的疗效。低大豆蛋白对高尿酸血症大鼠模型有肾保护作用。高大豆蛋白可升高高尿酸血症大鼠模型的血尿酸,并加重肾损害。 2.高大豆蛋白可升高高尿酸血症大鼠的血清甘油三酯。 3.低大豆蛋白对实验大鼠的营养状况无影响。
[Abstract]:Objective to study the feasibility of hyperuricemia rat model induced by different kinds of modeling agents and the effects of different concentrations of soybean protein on uric acid metabolism and renal function in hyperuricemia animal model. Methods in the first part, 20 male Sprague-Dawley rats were randomly divided into four groups. The rats were fed with 5% potassium oxazinate 10% yeast powder 10% yeast powder and 2% potassium oxazinate for 3 weeks. The body weight of rats was measured weekly, blood was collected, uric acid, creatinine, urea nitrogen, blood lipid and albumin were detected in urine samples. The second part: 20 rats with hyperuricemia were randomly divided into 4 groups. The rats were fed with 11% soybean protein 22% soybean protein 44% soybean protein and 22% soybean protein plus allopurinol for 6 weeks. Urine samples were measured for uric acid, creatinine, urea nitrogen, blood lipids and albumin. Results the first part: 1. Compared with the control group, the serum uric acid of rats in the 5% oxazinic acid group increased at the first 2 weeks, but decreased at the third week. The serum uric acid in the 10% yeast powder group increased only at the second week. The serum uric acid in the 2% oxazinic acid group increased at the 1st and 2nd week, but decreased at the third week. There was no statistical difference compared with the control group. There was no difference in serum uric acid level between the three model groups and the control group after 1 week. There was no significant difference in serum creatinine, urea nitrogen, albumin, body weight and uric acid between the model group and the control group. Part II: 1. Compared with the normal soybean protein group, the low soybean protein group and the normal soybean protein plus allopurinol group decreased the level of serum uric acid at the 6th week, and there was no significant difference between the low soybean protein group and the common soybean protein plus allopurinol group. The serum uric acid level of rats in the high soybean protein group was increased at the 5th week (P 0.05). Compared with the normal soybean protein group, the serum creatinine of rats in the low soybean protein group decreased P0.05N at the 6th week, the urea nitrogen decreased P0.05N at the 2nd week, and the serum creatinine and urea nitrogen levels of the rats in the high soybean protein group increased P0.055.3 at the 2nd week. Compared with the normal soybean protein group, the serum triglyceride of rats in the high soybean protein group was higher than that of the normal soybean protein group at the 2nd week, and the level of serum triglyceride in the high soybean protein group was higher than that in the normal soybean protein group. There was no significant difference in body weight, albumin, serum cholesterol, uric acid, urinary creatinine and urinary protein excretion. Conclusion 1. Continuous feeding of 5% potassium oxazinate can induce stable hyperuricemia, which can be used as a research model of hyperuricemia in the future. 2. 5% potassium oxazinate had no effect on the nutritional status and renal function of experimental rats. Part II: 1. Low soybean protein reduced serum uric acid in hyperuricemia rats, and its effect was equal to that of allopurinol. Low soybean protein has renal protective effect on hyperuricemia rat model. High soybean protein can increase serum uric acid in hyperuricemia rats and aggravate renal damage. 2. 2. High soybean protein can increase serum triglyceride in hyperuricemia rats. Low soybean protein had no effect on nutritional status of experimental rats.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R589.7;R-332

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