MSCTP实验动物模型研究

发布时间：2018-06-24 00:19

本文选题：动物模型 + 猪　；参考：《广西医科大学》2011年硕士论文

【摘要】：研究目的：探讨不同剂量、流速对比剂对动物门静脉达峰时间、门静脉强化峰值及门静脉成像的影响。材料与方法1、不同注射剂量、流速时门静脉达峰时间及强化峰值实验实验对象：选择正常巴马猪6头,体重约20kg-40kg。CT扫描前实验动物予以禁食10小时、禁水4小时及测量体重。其中4头猪分别进行不同剂量(2.0ml/kg,2.5ml/kg)和不同流速(2. Oml/s,2.5ml/s 3. Oml/s)配伍组合,扫描24次；另2头猪只进行3次上述不同剂量、不同流速配伍组合,扫描3次,共计扫描27次。同一头猪相隔扫描的时间至少超过48小时。CT扫描：正常巴马猪先行麻醉后,将其取俯卧位固定于自制纸板并放置于扫描床上,同时建立耳背静脉注射通道。扫描设备为64排64层GE Light Speed VCT。先行全肝脏CT平扫,选择层厚为5啪连续扫描。浏览图像并选取门脉主干(发出第一支门静脉右支层面或其向上2个层面)作为连续动态增强扫描(灌注扫描)的靶层面。经双筒高压注射器经耳背静脉团注离子型对比剂60%泛影葡胺,注射对比剂2s之后开始扫描,1层/次/秒,间隔1s,共扫描时间80s,获得图像40张。CT图像处理：将动态增强扫描数据传入GE Advantage Window4.4工作站,在所获得图像上,于门静脉主干放置ROI区,利用Perfusion4软件或手工测量并绘制门静脉的时间-密度曲线(TDC),观察门静脉达峰时间及其强化峰值。2、门静脉血管成像实验实验对象：上述实验中的4头巴马猪。实验方法：通过上述实验获得的门静脉达峰时间即最佳扫描延迟时间的基础上,对同一头巴马猪至少相隔48h后,用相同的流速、造影剂及剂量计算方式再次进行MSCT门静脉期增强扫描。扫描范围以包括整个肝实质。共获得24组MIP. VR图像。图像处理与分析：将扫描数据以1.25mmm薄层重建并传入GEAdvantage Window4.4工作站进行VR、MIP门静脉血管成像后处理。获得的门静脉血管图像进行质量评估,观察血管显示的分支情况、清晰度把门静脉血管图像分为A、B、C、D四个等级。所有计数指标输入SPSS16.0统计软件包(析因设计方差分析)及Microsoft Office Excel 2003进行统计学处理。结果：1、固定流速为2.0ml/s,剂量分别为2. Oml/kg及2.5ml/kg时,Imax P分别为187.9±13.3Hu,215.7±9.8Hu；固定流速为2.5m1/s,剂量分别为2. Oml/kg及2.5ml/kg时,Imax P分别为188.1±24.4Hu,227.3±36.7Hu；固定流速为3.0ml/s,剂量分别为2.0ml/kg及2.5ml/kg时,Imax P分别为214.5±17.OHu,235.3±11.5Hu。2、剂量不同时门静脉强化峰值有差别(P0.05)。在不考虑流速的前提下,剂量2.5ml/kg与2. Oml/kg相比,门静脉强化峰值增加了29.28HU(P0.05)。流速不同时门静脉强化峰值无统计学意义(P0.05)。3、固定流速为2.0ml/s,剂量分别为2.Oml/kg及2.5ml/kg时,Tmax P分别约为43.4±8.2 s,49.0±8.2s；固定流速为2.5ml/s,剂量分别为2.Oml/kg及2.5ml/kg时,Tmax P分别约为35.0±4s,39.5±6.4s；固定流速为3.0ml/s,以剂量分别为2. Oml/kg及2.5ml/kg时,Tmax P分别约为31.0±5.9 s,38.0±1.Os。4、不同流速及剂量对门静脉达峰时间均有影响(P0.05)。2.0ml/s与2.5m1/s及2.0ml/s与3.0ml/s之间有差别(P0.05),2.5ml/s与3.0ml/s之间无差别(p0.05)。在不考虑流速的前提下,剂量2.5m1/kg与2.0ml/kg相比,TmaxP延长5.73s(P0.05),无统计学意义。5、在剂量为2. Oml/kg情况下,流速为2.0ml/s时获得门脉评分为C级2例次,B级2例次；流速为2.5m1/s时获得门脉评分为B级2例次,A级2例次；流速为3.0ml/s时获得门脉评分为B级2例次,A级2例次。在剂量为2.5ml/kg情况下,流速为2.0ml/s时获得门脉评分为A级1例次,B级3例次；流速为2.5m1/s时获得门脉评分为A级3例次,B级1例次；流速为3.Oml/s时获得门脉评分为A级3例次,B级1例次。结论1、对比剂剂量的增加对门静脉强化峰值及门静脉达峰时间均有影响。其他参数不变时,随着剂量的增加,门静脉达峰时间延长,门静脉强化峰值提高。2、对比剂流速的提高对门静脉达峰时间有影响,对门静脉强化峰值无影响。其他参数不变时,随着流速的提高,门静脉达峰时间提前,门静脉强化峰值提高。3、在相同剂量情况下,流速为2.5m1/s与3.Oml/s时的MSCTP图像质量等级一致；剂量为2.Oml/kg与2.5m1/kg时,MSCTP图像质量大部分是B级以上。以达到诊断要求MSCTP的对比剂最低剂量、最低流速作为多层螺旋CT门静脉期扫描的推荐参数,本研究建议,对比剂剂量为2.0ml/kg、对比剂流速为2.5m1/s为佳。
[Abstract]:Objective : To investigate the effect of different dose and flow rate contrast agent on peak time of portal vein , peak value of portal vein and portal vein imaging . Oml/s,2.5ml/s 3. Oml / s combination , scanning for 24 times ;
The results were as follows : 1 layer / time / sec , interval 1s , scanning time 80s , scanning device is 64 rows of 64 - slice GE Light Speed vct . Image processing and analysis : The image of portal vein was reconstructed and passed into GEAdvantage Window4.4 workstation for VR and MIP portal vein imaging after image processing and analysis . The obtained portal vein image was evaluated by quality evaluation . The branch of vascular display was observed . The results were as follows : 1 . The fixed flow rate was 2.0ml / s and the dose was 2 . At Oml / kg and 2.5ml / kg , the difference was 187.9 卤 13.3Hu , 215.7 卤 9.8Hu , respectively .
The fixed flow rate was 2.5ml / s and the dose was 2 . When Oml / kg and 2.5ml / kg , the difference was 188.1 卤 24.4Hu , 227.3 卤 36.7Hu , respectively .
At the fixed flow rate of 3.0 ml / s and the dosage of 2.0ml / kg and 2.5ml / kg , respectively , the peak values were 214 . 5 卤 17 . OHu , 235 . 3 卤 11.5 Hu . 2 , and the dose did not differ significantly ( P 0 . 05 ) at the same time , but the dose was 2.5 ml / kg and 2 without considering the flow rate . Compared with Oml / kg , the peak value of portal vein enhancement increased 29.28HU ( P0.05 ) . The peak value of portal vein enhancement was 2.0ml / s , the dose was 2.0ml / kg and 2.5ml / kg , respectively , Tmax was 43.4 卤 8.2 s , 49.0 卤 8.2s , respectively .
At the fixed flow rate of 2.5ml / s and the dosage of 2.Oml / kg and 2.5ml / kg , Tmax was 33.0 卤 4s , 39.5 卤 6.4s , respectively ;
The fixed flow rate was 3.0 ml / s with a dose of 2 . At Oml / kg and 2.5ml / kg , Tmax was 31.0 卤 5.9 s , 38.0 卤 1.Os respectively . There was no difference between 2.0ml / s and 2.5ml / s and 2.0ml / s and 3.0ml / s ( P0.05 ) . In Oml / kg , the portal vein score was grade C in 2 cases and grade B in 2 cases when the flow rate was 2.0ml / s .
When the flow rate was 2.5ml / s , the portal vein score was grade B in 2 cases and grade A in 2 cases .
When the flow rate was 3.0ml / s , the portal vein score was grade B in 2 cases and grade A in 2 cases . At the dose of 2.5ml / kg , the portal vein score was Grade A and grade B was 3 cases .
When the flow rate was 2.5ml / s , the portal vein score was Grade A , 3 times and grade B was 1 case ;
The peak time of portal vein was prolonged and the peak value of portal vein was increased .
When the dose was 2.Oml / kg and 2.5ml / kg , the image quality of MSCTP was mostly higher than grade B . In order to reach the diagnostic requirement of MSCTP , the lowest dose and the lowest flow rate were the recommended parameters of multi - slice spiral CT portal vein scan . The study suggested that the contrast agent dose was 2.0ml / kg and the flow rate of contrast agent was 2.5ml / s .
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R816.5

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