山茱萸多糖对衰老模型大鼠学习记忆能力及海马突触可塑性的影响

发布时间：2018-06-24 02:32

本文选题：山茱萸多糖 + 衰老　；参考：《中国实验方剂学杂志》2016年13期

【摘要】：目的:观察山茱萸多糖对衰老模型大鼠学习记忆能力、海马脑源性神经生长因子(BDNF),酪氨酸蛋白激酶B(Trk B)蛋白表达及海马长时程增强(LTP)的影响,并探讨其机制。方法:清洁级SD大鼠40只,随机数字表法分为正常组、模型组、山茱萸多糖低、高剂量组,每组10只,采用D-半乳糖(140 mg·kg~(-1)·d-1)皮下注射构建大鼠衰老模型,同时正常组、模型组每天给予生理盐水、低剂量组和高剂量组给予山茱萸多糖(0.14,0.28 g·kg~(-1)·d-1)灌胃6周。Morris水迷宫检测学习记忆能力,高频刺激海马CA3区Schaffer侧支,在同侧海马CA1区诱导LTP的方法检测大鼠海马神经元突触可塑性的变化,免疫组化法检测海马神经元BDNF和Trk B蛋白的表达情况。结果:与正常组比较,模型组平均逃避潜伏期明显延长,在目标象限内游泳的距离占总距离的百分比和探索次数明显减少,海马LTP幅度显著降低,海马BDNF和Trk B蛋白表达降低(P0.01)。高剂量组与模型组比较平均逃避潜伏期明显降低,在目标象限内游泳的距离占总距离的百分比和探索次数明显升高,海马LTP幅度显著升高,海马BDNF和Trk B蛋白表达升高(P0.01)。结论:山茱萸多糖可通过提高海马BDNF和Trk B的表达及大鼠海马CA1区的LTP,明显改善突触的可塑性,这可能是山茱萸多糖提高学习记忆能力的重要机制之一。
[Abstract]:Aim: to observe the effects of Fructus Corni polysaccharides on learning and memory ability, expression of brain-derived nerve growth factor (BDNF), tyrosine protein kinase B (Trk B) and long-term potentiation (LTP) in hippocampus of aging rats. Methods: forty SD rats of clean grade were randomly divided into normal group, model group, low and high dose group of Cornus officinalis, 10 rats in each group. The aging model was established by subcutaneous injection of D-galactose (140 mg kg ~ (-1) d ~ (-1) into normal group. The model group was given normal saline daily, the low dose group and the high dose group were given the polysaccharides of Cornus officinalis (0.14 ~ 0.28 g kg ~ (-1) d ~ (-1) for 6 weeks. Morris water maze was used to detect the learning and memory ability, and the Schaffer side branch of hippocampal CA3 region was stimulated by high frequency. The changes of synaptic plasticity in hippocampal neurons and the expression of BDNF and Trk B protein in hippocampal neurons were detected by immunocytochemical method in the ipsilateral hippocampal CA1 region. Results: compared with the normal group, the average escape latency of the model group was significantly prolonged, the percentage of swimming distance in the target quadrant and the number of explorations were significantly decreased, the amplitude of hippocampal LTP was significantly decreased, and the expression of BDNF and Trk B protein was decreased (P0.01). Compared with the model group, the average escape latency in the high dose group decreased significantly, the percentage of swimming distance in the target quadrant and the number of explorations increased significantly, the amplitude of hippocampal LTP increased significantly, and the expression of BDNF and Trk B protein in the hippocampus increased (P0.01). Conclusion: Fructus Corni polysaccharides can improve synaptic plasticity by increasing the expression of BDNF and Trk B in hippocampus and LTPin hippocampal CA1 area of rats, which may be one of the important mechanisms of enhancing learning and memory ability of Cornus officinalis polysaccharide.
【作者单位】：南阳医学高等专科学校;
【基金】：南阳市科技攻关计划项目(2012GG056)
【分类号】：R285.5;R-332

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