酶联免疫吸附试验在丙型肝炎抗体检测中质量控制探讨

发布时间：2018-06-24 03:35

本文选题：丙型肝炎病毒 + 酶联免疫吸附试验　；参考：《现代免疫学》2017年06期

【摘要】：寻找适合酶联免疫吸附试验对丙型肝炎抗体检测的室内质量控制(internal quality controlling,IQC)的方法。采用Levey-Jennings质控图法、即刻法、加强即刻法(CV30%)对相同的20例数据进行处理。再用双质控法处理更换试剂批号和血清质控。和传统的质控方法比较,Levey-Jennings质控图法和即刻法对数据监控上均存在假失控和假在控。而加强即刻法显示良好效果。配合双质控法处理更换试剂批号和血清质控也很优秀。依据提出的质控方法进行室内质控,其当年室间质评结果全部合格。课题组的结论是控制变异系数的加强即刻法配合L-J质控图法更适用于酶联免疫吸附试验的IQC。
[Abstract]:To find a suitable method for the detection of hepatitis C antibody by enzyme linked immunosorbent assay (Elisa). Levey-Jennings quality control chart method, immediate method and enhanced instant method (CV30%) were used to process the same 20 cases of data. Then the batch number of the replacement reagent and the serum quality control were treated by double quality control method. Compared with the traditional quality control method, Levey-Jennings quality control chart method and instant control method have false runaway and false control. The enhancement of the immediate method showed good results. Combined with double quality control method to deal with the replacement reagent batch number and serum quality control is also very good. The indoor quality control was carried out according to the proposed quality control method, and the results of indoor quality assessment were all up to standard in that year. The conclusion of our research group is that the enhanced immediate method of controlling coefficient of variation and L-J quality control map method are more suitable for IQC of enzyme linked immunosorbent assay (Elisa).
【作者单位】：江西省萍乡市人民医院检验科;
【分类号】：R392-33

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