肢体缺血性再灌注损伤的新型动物模型制作

发布时间：2018-06-25 08:14

本文选题：充气止血带 + 肢体　；参考：《新疆医科大学》2012年硕士论文

【摘要】：目的：用充气止血带制作肢体缺血再灌注损伤的新型动物模型，研究其对周围神经和骨骼肌损伤的影响。方法：选择健康新西兰大白兔6月龄，30只，体重3.5±0.3Kg，雌雄不限，随机分为3组，每组10只：A组：对照组，B组：缺血2小时，C组：缺血4小时。在家兔左侧后肢环扎充气止血带，于不同时间点松开，造成左侧后肢缺血再灌注损伤的模型。对照组不扎充气止血带，于第1、2、3、4、5、6小时检测肢体的神经电生理学，B组、C组于再灌注（松开止血带，血供恢复后）的1、2、3、4、5、6小时检测肢体的神经电生理学，A组于第6小时观察骨骼肌的形态学，B组、C组于再灌注（松开止血带，血供恢复后）的第6小时观察骨骼肌的形态学，每组于术后第5天观察、评估左侧后肢的行走功能。结果：随着缺血时间的延长、再灌注的损伤，B组、C组和A组相比较，周围神经的潜伏期延长、波幅降低，，传导速度降低，三组之间的潜伏期、波幅、传导速度差异均有统计学意义（P＜0.05），光镜观察骨骼肌可见（B组和C组）：横纹紊乱、肌纤维断裂、间质血管扩张充血、大量中性粒细胞浸润。结论：经过缺血期和再灌注损伤的交互作用后，肢体的功能性损伤进一步加重，出现了不可逆的病损。该模型制作对动物的损伤较小，更贴近临床。
[Abstract]:Aim: to study the effects of inflatable tourniquet on peripheral nerve and skeletal muscle injury. Methods: 30 healthy New Zealand white rabbits were randomly divided into 3 groups (n = 10): control group (n = 2): group C: ischemia (n = 4). The inflatable tourniquet was ligated on the left hind limb of the rabbit and released at different time points to make the model of ischemia reperfusion injury of the left hind limb. The control group did not tie an inflatable tourniquet, and the limbs were examined for neuroelectrophysiology at 6 hours at 1, 2, 3, 4, 5 and 6 hours after reperfusion (loosening the tourniquet) in group B and C, respectively. 6 hours after recovery of blood supply, the morphology of skeletal muscle was observed in group A at 6 hours and the morphology of skeletal muscle in group C at 6 hours after reperfusion (release of tourniquet and recovery of blood supply). The walking function of left hindlimb was evaluated 5 days after operation in each group. Results: with the prolongation of ischemic time, compared with group A, the latency, amplitude and conduction velocity of peripheral nerve in group B were prolonged, and the latency and amplitude of wave were decreased between the three groups. The difference of conduction velocity was statistically significant (P < 0.05). The skeletal muscle was observed under light microscope (group B and C): transverse striation, broken muscle fiber, hyperemia of interstitial vessels and infiltration of neutrophils. Conclusion: after the interaction between ischemia and reperfusion injury, the functional injury of limbs is further aggravated and irreversible lesions appear. The model has less damage to animals and is closer to clinical practice.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R658;R-332

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