尿源性脓毒血症兔模型的制备及乌司他丁的治疗作用

发布时间：2018-06-25 09:16

本文选题：家兔 + 乌司他丁　；参考：《南华大学》2012年硕士论文

【摘要】：目的将脂多糖(lipopolysaccharide, LPS)注入输尿管内并结扎该侧输尿管建立尿源性脓毒血症的兔模型；探讨乌司他丁对脓毒血症的疗效,为乌司他丁治疗尿源性脓毒血症提供理论和实际依据。方法 1、选择结扎家兔左侧输尿管,左侧输尿管内注入脂多糖,建立尿路梗阻及泌尿系感染并脓毒症的动物模型。 2、将24只健康雄性家兔随机分成四组,每组6只。正常组(Xormal组),假手术组(Sham组),模型组(Model组)和治疗组(UTI组)。 3、造模36h后监测各组实验家兔体温,呼吸频率,检测外周血细胞计数、血清C反应蛋白；采用ELISA法测定血清TNF-α、IL-10的含量；各组肝、肾、肺组织行HE染色,光学显微镜下观察各组织的形态结构变化；免疫组织化学检测各组家兔肾组织的TNF-α、IL-10蛋白的表达水平。结里 1、家兔的一般情况实验家兔假手术组与正常组体温,呼吸频率变化不明显,无统计学意义(P0.05)；模型组明显高于正常组及假手术组(P0.05),乌司他丁组低于模型组(P0.05)。 2、血液检测指标血细胞WBC计数结果：实验家兔正常组与假手术组比较,差别无统计学意义(P0.05)；模型组明显高于正常组及假手术组(P0.05),治疗组有下降趋势,有统计学意义(P0.05)。各组血细胞计数(PLT)结果,差异无统计学意义(P().05)。血清C-反应蛋白(CRP)结果：实验家兔假手术组有升高趋势,与正常组比较差异有统计学意义(P0.05)；模型组明显升高,差别有统计学意义(P0.05)；治疗组与模型组比较明显下降,差别有统计学意义(P0.05)。家兔血清TNF-α, IL-10含量结果：与正常组比较,假手术组家兔血清TNF-α IL-10变化无统计学意义(P0.05)；与假手术组比较,模型组明显升高(P0.05)；模型组与治疗组比较,治疗组血清TNF-α明显降低(P0.05),血清IL-10明显升高(P0.05)。 3、肝,肾,肺HE染色实验家兔正常组和假手术组组织形态结构正常。模型组,肝细胞轻微肿胀,胞浆松弛,有散在炎症细胞浸润；肾小球变形,肾小管管腔变大,肾间质有炎症细胞浸润；肺泡间隔弥漫性增厚,有炎症细胞浸润。治疗组较模型组上述变化有所减轻。 4. TNF-α,IL-10蛋白免疫组化家兔正常组、假手术组肾组织内TNF-α,IL-10蛋白主要呈微弱表达；TNF-α蛋白在模型组表达最强；IL-10蛋白在治疗组表达最强。结论 1、应用内毒素(LPS)注入输尿管,并结扎该侧输尿管36小时后基本能成功复制尿源性脓毒症模型。 2、乌司他丁能有效防治尿源性脓毒症,减轻组织器官的炎症反应,其机理可能是通过下调TNF-α,上调IL-10。
[Abstract]:Objective to establish a rabbit model of ureteral sepsis by injecting lipopolysaccharide (LPS) into the ureter and ligating the ureter, and to investigate the efficacy of ulinastatin in the treatment of sepsis. To provide theoretical and practical basis for the treatment of urinal sepsis with ulinastatin. Methods 1. The animal model of urinary tract obstruction and urinary tract infection with sepsis was established by ligating the left ureter and injecting lipopolysaccharide into the left ureter. 2Twenty four healthy male rabbits were randomly divided into four groups. There were 6 rats in each group. Normal group (Xormal group), sham-operated group (Sham group), model group (model group) and treatment group (UTI group) .3The temperature, respiratory rate, peripheral blood cell count and serum C-reactive protein were monitored after 36 hours of modeling. Serum TNF- 伪 IL-10 was determined by Elisa, liver, kidney and lung tissues were stained with HE, morphological and structural changes of each tissue were observed under optical microscope, and the expression of TNF- 伪 IL-10 protein in renal tissue of rabbits in each group was detected by immunohistochemistry. The general situation of rabbits the temperature and respiratory frequency of the sham operation group and the normal group were not significantly changed (P0.05). Model group was significantly higher than normal group and sham-operation group (P0.05), ulinastatin group was lower than model group (P0.05). Model group was significantly higher than normal group and sham-operation group (P0.05), treatment group had a downward trend, there was statistical significance (P0.05). There was no significant difference in blood cell count (PLT) in each group (P (.05). Serum C-reactive protein (CRP) results: experimental rabbits sham-operation group had an increasing trend, compared with the normal group, the difference was statistically significant (P0.05); the model group significantly increased, the difference was statistically significant (P0.05); treatment group and model group compared with the model group significantly decreased, The difference was statistically significant (P0.05). Results of serum TNF- 伪, IL-10 levels in rabbits: compared with the normal group, the changes of serum TNF- 伪 IL-10 in the sham operation group had no statistical significance (P0.05); compared with the sham operation group, the serum TNF- 伪 IL-10 level in the model group was significantly higher (P0.05); the model group and the treatment group were significantly higher than those in the treatment group. In treatment group, serum TNF- 伪 was significantly decreased (P0.05), serum IL-10 was significantly increased (P0.05). 3. Liver, kidney, lung HE staining of normal rabbits and sham-operation group were normal. In the model group, the hepatocytes were slightly swollen, cytoplasm relaxed, inflammatory cell infiltration scattered; glomerular deformation, tubule lumen becoming larger, interstitial inflammatory cell infiltration; alveolar septum diffuse thickening, inflammatory cell infiltration. Compared with the model group, the above changes in the treatment group were alleviated. 4. 4. The expression of TNF- 伪 and IL-10 protein in renal tissue of sham-operated group was mainly weak. The expression of TNF- 伪 protein in the model group was the strongest, and the expression of IL-10 protein was the strongest in the treatment group. Conclusion 1. Ulinastatin can effectively prevent and treat ureteral sepsis 36 hours after lipopolysaccharide (LPS) injection and ligation of ureter. The mechanism of reducing inflammation in tissues and organs may be through down-regulating TNF- 伪 and upregulating IL-10.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R459.7;R-332

【引证文献】