HB-H-8树脂吸附低密度脂蛋白的实验研究

发布时间：2018-06-26 05:25

  本文选题：低密度脂蛋白 + 高密度脂蛋白　； 参考：《天津医科大学》2012年硕士论文

【摘要】：目的：高脂血症对健康有很大的负面影响,受其长期作用血管可发生动脉粥样硬化(atheoselorsis, As),而动脉粥样硬化是导致心脑血管疾病的重要原因,其发生与食物、生活习惯、遗传等多种因素有关,研究证明人体血液中低密度脂蛋白(low density lipoprotein, LDL)的升高以及由此而引起LDL进入血管壁中并被氧化成氧化态的低密度脂蛋白(Ox-LDL)是导致As的主要原因。另一方面,高密度脂蛋白(high density lipoprotein, HDL)能阻止As的形成及加重。如果能有效控制血中LDL水平而又不引起HDL的损失,不仅能防止As的发生,而且对已形成的As有明显减轻作用,进而有利于心脑血管疾病的防治。目前,用于降低血液中低密度脂蛋白(low density lipoprotein, LDL)的吸附分离材料尚存在需要改进的地方。设计合成出预计对低密度脂蛋白有较好吸附效果的HB-H-8树脂,通过体外实验探讨HB-H-8树脂吸附低密度脂蛋白的有效性和特异性。 方法：HB-H-8树脂与其他吸附材料的比较实验：将合成的HB-H-8树脂与其他常用吸附材料进行对比实验,这些吸附材料包括阴离子交换树脂,阳离子交换树脂,弱极性树脂,日本炭化树脂,海藻酸钠固定化多粘菌素B和大孔吸附树脂。通过对20例LDL高于正常范围的患者血清标本进行体外静态吸附实验,测定7种吸附材料吸附前后LDL浓度,计算其吸附率,比较实验结果。HB-H-8树脂对LDL吸附性能的研究：HB-H-8树脂经过处理之后使用其对LDL高于正常范围的患者血清标本进行体外动态考察HB-H-8的吸附饱和时间以及温度对HB-H-8吸附效果的影响；使用HB-H-8进行静态吸附实验,测定血清中低密度脂蛋白浓度(1owdensity lipoprotein, LDL)高密度脂蛋白(high density lipoprotein, HDL)总胆固醇(cholesterol total, CHO),甘油三酯(triglyceride, TG)吸附前后浓度变化,计算吸附率考察HB-H-8的吸附特异性。 结果：7种吸附材料吸附LDL比较实验结果表明HB-H-8树脂对LDL平均吸附率为63.2%,其吸附能力高于其他6种吸附树脂。HB-H-8体外动态吸附结果表明,HB-H-8树脂对低密度脂蛋白(LDL)的吸附饱和时间为2个小时,最适吸附温度为恒温37℃,体外静态吸附结果显示HB-H-8树脂对LDL平均吸附率为63.2%,而对高密度脂蛋白(high density lipoprotein, HDL)的平均吸附率为1.9%。 结论：HB-H-8树脂对LDL具有良好的特异性吸附性能,有望开发为一种低密度脂蛋白的血液净化医用吸附材料。
[Abstract]:Objective: hyperlipidemia has a great negative effect on health. Atherosclerosis is an important cause of cardiovascular and cerebrovascular diseases, and it is associated with food and living habits. Genetic analysis and other factors have shown that the increase of low density lipoprotein (low density lipoprotein,) in human blood and the oxidized low density lipoprotein (Ox-LDL) into the vascular wall are the main causes of as. High density lipoprotein (HDL), on the other hand, prevents the formation and aggravation of as. If the level of LDL in blood can be effectively controlled without causing HDL loss, it can not only prevent the occurrence of as, but also alleviate the formation of as, which is beneficial to the prevention and treatment of cardiovascular and cerebrovascular diseases. At present, there is still room for improvement in adsorptive separation materials used to reduce low density lipoprotein (low density lipoprotein,) in blood. HB-H-8 resin, which is expected to adsorb low density lipoprotein, was designed and synthesized. The effectiveness and specificity of HB-H-8 resin in adsorption of low density lipoprotein were investigated by experiments in vitro. Methods compared with other adsorption materials, the synthesized HB-H-8 resin was compared with other commonly used adsorption materials, including anion exchange resin, cation exchange resin, weakly polar resin. Japanese carbonized resin, sodium alginate immobilized polymyxin B and macroporous resin. In vitro static adsorption experiments were carried out on 20 serum samples of patients with LDL above normal range. The concentration of LDL was measured before and after the adsorption of 7 kinds of adsorption materials, and the adsorption rate was calculated. Study on the adsorption performance of HB-H-8 resin on LDL after treatment, the effect of temperature on the adsorption of HB-H-8 on HB-H-8 was investigated dynamically in vitro by using HB-H-8 resin in serum samples of patients whose LDL was higher than normal range. HB-H-8 was used to carry out static adsorption experiment. The concentrations of (cholesterol total, Cho and triglyceride (TG) in serum of 1owdensity lipoprotein (LDL-C), high density lipoprotein (high density lipoprotein,) and triglyceride (TG) were determined before and after adsorption. The adsorption specificity of HB-H-8 was investigated by calculating the adsorption rate of HB-H-8. Results the average adsorption rate of LDL on HB-H-8 resin was 63.2%, and the adsorption ability of HB-H-8 resin was higher than that of other six kinds of adsorption resin. HB-H-8 resin showed that HB-H-8 resin could adsorb low density lipoprotein (LDL) in vitro. The adsorption saturation time is 2 hours. The optimum adsorption temperature was 37 鈩，

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