人正常上皮细胞的原代培养

发布时间：2018-06-26 07:09

本文选题：人正常上皮细胞 + 上皮组织　；参考：《中南大学学报(医学版)》2017年11期

【摘要】：传统的人正常上皮细胞原代培养方法存在培养率低、培养出的细胞活性差、操作繁琐等缺点,为了解决这些问题,近年来国内外的众多研究者对人正常上皮细胞的原代培养过程进行了大量研究和不断改进。笔者主要综述人正常上皮细胞分离纯化的一些方法(如组织块分离法、酶消化分离法、机械刷取法、红细胞裂解法、Percoll分层液密度梯度分离法等)以及培养传代中可应用到的一些方法(如无血清培养基联合低浓度血清培养基培养法、鼠尾胶原包被法、玻璃培养瓶联合塑料培养皿培养法);其次阐述了人正常上皮细胞的生物学特性及免疫细胞化学染色法、台盼蓝拒染法等生物学性状检测的方法,并且对培养中无菌操作、培养时和分离时细胞外环境的条件、差速贴壁的次数、添加剂的选择与用量等影响因素作了归纳。
[Abstract]:In order to solve these problems, the traditional method of primary culture of human normal epithelial cells has the disadvantages of low culture rate, poor cell activity and complicated operation. In recent years, many researchers at home and abroad have done a lot of research and improvement on the primary culture process of human normal epithelial cells. This paper reviews some methods of isolation and purification of human normal epithelial cells (such as tissue mass separation, enzyme digestion, mechanical brush extraction, etc.) RBC cleavage method, Percoll stratified liquid density gradient method, etc.) and some methods that can be used in culture passage (such as serum-free medium combined with low concentration serum medium culture method, rat tail collagen coating method, etc. The biological characteristics of human normal epithelial cells and the detection methods of immunocytochemical staining, trypan blue exclusion, etc. The factors affecting the extracellular environment during culture and separation, the number of different adhesions, the selection and dosage of additives were summarized.
【作者单位】：湘南学院临床医学系;湘南学院基础医学院;湘南学院病理学研究所;
【基金】：湖南省自然科学基金(2016JJ6143) 湖南省卫生和计划生育委员会支持项目(C2016048) 湘南学院一般项目(2015XB19)~~
【分类号】：Q813.11;R329.2

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