幽门螺杆菌感染小鼠慢性胃炎模型的建立及评价

发布时间：2018-06-26 13:48

本文选题：幽门螺杆菌 + 慢性胃炎　；参考：《中国比较医学杂志》2017年07期

【摘要】：目的建立幽门螺杆菌(H.pylori)感染动物模型,评价H.pylori相关慢性胃炎胃黏膜病理变化。方法灌胃H.pylori SS1菌株建立在体感染,感染后第2周后采用快速尿素酶法和PCR法检测感染成功率;确认感染成功后再分别继续饲养至6周和12周,建立H.pylori相关慢性胃炎动物模型。实验结束后,取胃腺体组织别进行HE和硼酸美兰染色,分析胃炎程度及H.pylori感染程度;生化法检测胃组织中髓过氧化物酶(myeloperoxidase,MPO),超氧化物歧化酶(superoxide dismutase,SOD),丙二醛(malondialdehyde,MDA)和过氧化氢酶(catalase,CAT)的含量变化;RT-q PCR法检测胃组织中COX-2、iNOS、TNF-α和IL-1β基因表达的变化。结果与正常组相比,模型6周和12周组胃组织内可见H.pylori定植,并且黏膜层有不同程度的慢性炎性细胞浸润,腺体萎缩和肠化生情况;同时组织中CAT和SOD的含量明显下降,MPO和MDA的水平和COX-2、iNOS、TNF-α和IL-1β基因表达均有显著上升(P0.05或P0.01)。结论通过灌胃给菌的方法可以成功将H.pylori定植于小鼠体内,并在定植的6周和12周后均可引起慢性炎性细胞的浸润,并使增强胃腺体组织内氧化应激水平和促炎基因的表达,但12周模型感染程度更深,出现腺体萎缩和肠化生的情况。
[Abstract]:Objective to establish an animal model of Helicobacter pylori (H. pylori) infection and to evaluate the gastric mucosal pathological changes in H. pylori associated chronic gastritis. Methods H.pylori SS1 strain was perfused to establish in vivo infection. The success rate of infection was detected by rapid urease method and PCR method 2 weeks after infection, and the animal model of H. pylori related chronic gastritis was established by continuing to feed for 6 weeks and 12 weeks after successful infection. At the end of the experiment, the gastric glands were stained with HE and methylene borate to analyze the degree of gastritis and H.pylori infection. The contents of myeloperoxidase (MPO), superoxide dismutase (superoxide), malondialdehyde (malondialdehyde) and catalase (cat) in gastric tissues were detected by biochemical method. Results H.pylori colonization was observed in gastric tissue of 6 and 12 weeks of model compared with normal group, and chronic inflammatory cell infiltration, atrophy of glands and intestinal metaplasia were observed in mucosal layer. At the same time, the contents of cat and SOD decreased significantly (P0.05 or P0.01), the levels of MPO and MDA and the expression of TNF- 伪 and IL-1 尾 increased significantly (P0.05 or P0.01). Conclusion H.pylori can be successfully colonized in mice by intragastric administration of H. pylori, which can induce chronic inflammatory cell infiltration at 6 and 12 weeks after implantation, and enhance the level of oxidative stress and the expression of pro-inflammatory gene in gastric glands. At 12 weeks, however, the model was more infected, with atrophy of glands and intestinal metaplasia.
【作者单位】：广州中医药大学中药学院;
【基金】：国家自然科学基金资助项目(编号:81374043) 广东省科技计划资助项目(编号:2016A020217019) 广州市科技计划资助项目(编号:201607010336) 广东省省级科技计划项目(编号:2013A022100001)
【分类号】：R-332;R573.3

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