卵清蛋白致敏小鼠嗜酸细胞性胃肠炎模型的制备

发布时间：2018-06-27 02:20

本文选题：嗜酸细胞 + 胃肠炎　；参考：《临床儿科杂志》2016年04期

【摘要】：目的建立卵清蛋白致敏小鼠嗜酸细胞性胃肠炎模型。方法 6周龄BALB/c雌性小鼠20只,随机分为对照组及模型组。第1天模型组用卵清蛋白(OVA)与氢氧化铝[AL(OH)3]凝胶腹腔注射基础致敏,第15天模型组用OVA/生理盐水腹腔注射强化致敏,对照组均用等量生理盐水腹腔注射;第18~30天模型组隔日用聚乳酸-羟基乙酸共聚物(PLGA)包裹的OVA与生理盐水混匀灌胃激发,共7次,对照组用等量生理盐水灌胃。造模后检测粪便隐血,计算活动指数评分(DAI);第31天处死小鼠,检测血清嗜酸性粒细胞(EOS),分别取胃、回肠、结肠等组织,光镜下观察组织病理变化及EOS密度。结果模型组小鼠在灌胃激发后均出现竖毛、弓背、懒动等表现,两组体质量变化无统计学差异,模型组DAI评分较对照组明显增高,差异有统计学意义(P0.01);两组外周血EOS计数差异无统计学意义(P0.05);光镜下胃肠黏膜EOS密度模型组较对照组增高,差异有统计学意义(P0.05);光镜下模型组小鼠胃肠黏膜损伤明显。结论致敏原OVA联合免疫佐剂氢氧化铝通过腹腔注射基础致敏及强化致敏,然后用PLGA包裹的OVA颗粒连续隔日灌胃7次作激发,可以成功制备嗜酸细胞性胃肠炎小鼠动物模型。
[Abstract]:Objective to establish eosinophilic gastroenteritis model in ovalbumin sensitized mice. Methods Twenty BALB / c female mice aged 6 weeks were randomly divided into two groups: control group and model group. On day 1, the model group was sensitized with ovalbumin (OVA) and aluminum hydroxide [AL (OH) 3] gel on the basis of intraperitoneal injection. On the 15th day, the model group was sensitized by OVA / normal saline, and the control group was injected with the same amount of saline. The rats in the model group were treated with polylactic acid-glycolic acid copolymer (PLGA) -coated OVA and normal saline for 7 times on the 1830 th day, and the control group was given the same amount of normal saline. After modeling, fecal occult blood was detected, activity index (Dai) was calculated, serum eosinophil (EOS) was detected on the 31st day, gastric, ileal and colonic tissues were taken, histopathological changes and EOS density were observed under light microscope. Results all the mice in the model group showed vertical hair, arch back and lazy movement after gastric stimulation. There was no significant difference in body mass between the two groups. The Dai score in the model group was significantly higher than that in the control group. The difference was statistically significant (P0.01); there was no significant difference in EOS count in peripheral blood between the two groups (P0.05); the EOS density of gastrointestinal mucosa in the model group was higher than that in the control group under light microscope (P0.05); the injury of gastrointestinal mucosa in the model group was obvious under light microscope (P0.05). Conclusion the animal model of eosinophilic gastroenteritis can be successfully established by injecting the allergen OVA combined with immune adjuvant aluminum hydroxide by intraperitoneal injection of basic and enhanced sensitization and then using PLGA encapsulated OVA granules for 7 consecutive days to induce eosinophilic gastroenteritis in mice.
【作者单位】：深圳市妇幼保健院;广州市妇女儿童医疗中心;
【基金】：广东省医学科研基金(No.B2015043)
【分类号】：R-332;R725.7

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