C型利钠肽基因调控斑马鱼胚胎血管发育

发布时间：2018-07-06 12:30

本文选题：nppc + 原位杂交　；参考：《生理学报》2017年01期

【摘要】：本研究旨在探索C型利钠肽前体蛋白基因(natriuretic peptide precursor C,nppc)在斑马鱼胚胎期的表达及其在血管发育过程中的功能。利用胚胎整体原位杂交技术检测nppc基因在斑马鱼胚胎期的表达。同时使用转基因斑马鱼系Tg(flk1:GFP)和Tg(fli1a:n GFP),显微注射nppc特异性吗啉基反义寡核苷酸(morpholino)和nppc m RNA调控nppc基因表达,利用激光共聚焦显微镜观察分析斑马鱼节间血管(intersegment vessel,ISV)表型,并统计内皮细胞数目。结果显示,在受精后24 h和48 h,nppc基因在斑马鱼脑、心脏、血管系统中都有表达。下调nppc基因表达导致ISV发育缺陷,ISV内皮细胞数目减少。以上结果表明下调nppc基因表达可能通过抑制内皮细胞增殖和内皮细胞迁移调控斑马鱼胚胎血管发育。
[Abstract]:The purpose of this study was to investigate the expression of C-type natriuretic peptide precursor protein gene (natriuretic peptide precursor Cnppc) in zebrafish at embryonic stage and its function in vascular development. The expression of nppc gene in zebrafish embryos was detected by in situ hybridization. The expression of nppc gene was regulated by microinjection of nppc specific morpholinyl antisense oligonucleotide (morpholino) and nppc m RNA using transgenic zebrafish lines TG (flk1: GFP) and TG (fli1a:n GFP). The phenotypes of intersegment vessels were observed and analyzed by confocal laser microscopy. And count the number of endothelial cells. The results showed that the nppc gene was expressed in the brain, heart and vascular system of zebrafish at 24 h and 48 h after fertilization. Down-regulation of nppc gene expression resulted in a decrease in the number of ISV endothelial cells. These results suggest that down-regulation of nppc gene expression may regulate the angiogenesis of zebrafish embryos by inhibiting endothelial cell proliferation and endothelial cell migration.
【作者单位】：广东医科大学附属医院;南通大学神经再生重点实验室;
【基金】：supported by grants from the National Natural Science Foundation of China(No.31370824) the Special Support Plan of Excellent Young Talents,Guangdong Province,China(No.[2015]8) Initial Program of Excellent Young Teachers in Colleges and Universities in Guangdong Province,China(No.[2016]6:YQ2015081)
【分类号】：R3416

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