寒凝血瘀型消化性溃疡大鼠模型建立及胃黏膜血流量、血流变学观察

发布时间：2018-07-06 19:21

本文选题：寒凝血瘀型 + 消化性溃疡　；参考：《辽宁中医药大学》2011年硕士论文

【摘要】：目的:建立寒凝血瘀型消化性溃疡大鼠模型,为临床研究提供实验模型。材料与方法: 20只大鼠随机分为2组,模型组(10只),采用改良冷暴露法,冷暴露同时,给予吲哚美辛,造成寒凝血瘀型消化性溃疡模型,对照组(10只),不施加冷暴露处理,肉眼及光镜观察大鼠症状、体征及胃粘膜溃疡情况,同时检测胃粘膜微循环血流量及血流变学改变。结果: 模型组大鼠冷冻后出现蜷缩少动、反应迟钝、寒战,喜扎堆,呼吸微弱,被毛无光泽,饮水量少,小便色清,大便湿烂,爪尾部紫暗,耳色暗红等症状,光镜下可见胃黏膜有明显损伤,条索状糜烂,以胃体部为主,整个黏膜层细胞有明显变性、胃小凹消失;主细胞、粘液细胞肿大,坏死或脱落,固有膜有灶性出血及急性炎症反应,损伤穿透黏膜层。对照组大鼠精神良好,动作敏捷有力,摄食正常,被毛密泽,紧贴身体,体肌健壮,胃黏膜基本正常。模型组胃粘膜溃疡指数(11.55±2.74)明显高于对照组(P0.01)。模型组血液流变学指标亦较对照组升高(P0.01)。模型组胃粘膜微循环血流量(57.18±7.12)明显少于对照组(87.06±3.40)(P0.01)。结论: 采用改良冷暴露联合吲哚美辛法建立的寒凝血瘀型消化性溃疡大鼠模型,符合中医证侯及消化性溃疡“病症结合”的特点,适合中医学实验研究。
[Abstract]:Objective: to establish a rat model of peptic ulcer with cold coagulation and blood stasis, and to provide an experimental model for clinical study. Materials and methods: twenty rats were randomly divided into two groups: the model group (n = 10) and the control group (n = 10). The symptoms, signs and gastric mucosal ulcers were observed with naked eye and light microscope. The blood flow and hemorheology of gastric mucosal microcirculation were also detected. Results: in the model group, the rats were curled up and moved, slow in reaction, shivering, faint in breathing, dull in hair, less in drinking water, clear in urine, wet in stool, purple in the tail of claw, dark red in ear, and so on. Under the light microscope, the gastric mucosa was obviously damaged, striped erosion, mainly in the body of the stomach, the cells of the whole mucosal layer were obviously denatured, the fovea of the stomach disappeared, the main cells, the mucous cells were enlarged, necrotized or shedded, The lamina propria has focal hemorrhage and acute inflammatory reaction, which damages and penetrates the mucosal layer. The rats in the control group were in good spirits, quick and strong, normal food intake, Mao Mizawa, close to the body, strong body muscles and normal gastric mucosa. The gastric mucosal ulcer index in the model group (11.55 卤2.74) was significantly higher than that in the control group (P0.01). The hemorheological indexes in the model group were also higher than those in the control group (P0.01). The blood flow of gastric mucosal microcirculation in the model group (57.18 卤7.12) was significantly lower than that in the control group (87.06 卤3.40) (P0.01). Conclusion: the model of peptic ulcer of cold coagulation and blood stasis type was established by modified cold exposure combined with indomethacin method, which accords with the characteristics of TCM syndromes and peptic ulcer "disease combination", and is suitable for the experimental study of traditional Chinese medicine.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332

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