痰瘀同治法干预咳嗽变异性哮喘大鼠病症结合动物模型气道慢性炎症疗效的实验研究

发布时间：2018-07-09 19:14

本文选题：咳嗽变异性哮喘 + 大鼠　；参考：《中华中医药学刊》2017年01期

【摘要】：目的:观察痰瘀同治法改善咳嗽变异性哮喘(CVA)大鼠病症结合动物模型气道慢性炎症的疗效。方法:将72只大鼠分为正常对照组、模型对照组、激素吸入组、清肺化痰组、活血化瘀组、痰瘀同治组,制备咳嗽变异性哮喘痰瘀互结结合大鼠模型,灌胃给药15天,观察各组大鼠一般状态、中医证候、肺支气管病理组织形态、支气管肺泡灌洗液(BALF)嗜酸性粒细胞计数及测定血清Ig E水平的变化。结果:与正常对照组比较,模型组CVA大鼠支气管管腔狭窄,黏膜增厚,支气管壁及可见大量嗜酸性粒细胞浸润,BALF涂片EOS计数显著升高,血清中Ig E的含量显著升高,与正常对照组比较均有显著性差异(P0.01)。激素治疗组及各中药治疗组,肺支气管病理、EOS计数及血清Ig E含量均有显著改善,与模型组比较有显著性差异(P0.01),以激素治疗组及肝肺同治组指标改善最为显著。痰瘀同治法可以明显改善模型大鼠气滞血瘀、痰瘀互结的中医证候,改善大鼠一般状态。结论:痰瘀同治法可改善痰瘀互结的中医证候,降低CVA模型大鼠慢性气道炎症反应,调整免疫异常。
[Abstract]:Objective: to observe the effect of phlegm and blood stasis therapy on chronic airway inflammation in cough variant asthma (CVA) rats. Methods: Seventy-two rats were divided into normal control group, model control group, hormone inhalation group, phlegm clearing and phlegm removing group, activating blood circulation and removing blood stasis group, and phlegm and blood stasis treatment group. The model of cough variant asthma combined with phlegm and blood stasis was established, and the rats were given orally for 15 days. The general state, TCM syndrome, pathological morphology of lung bronchus, eosinophilic granulocyte count in bronchoalveolar lavage fluid (BALF) and serum IgE level were observed. Results: compared with the normal control group, the bronchial lumen stenosis, mucosal thickening, eosinophil infiltration in bronchial wall and EOS count in BALF smear of CVA rats in model group were significantly increased, and the content of IgE in serum was significantly increased. There was significant difference compared with the normal control group (P0.01). The EOS count and serum IgE content of lung bronchus pathology in hormone treatment group and traditional Chinese medicine treatment group were significantly improved compared with model group (P0.01), especially in hormone treatment group and hepatopulmonary treatment group. Phlegm and blood stasis treatment can obviously improve the Qi stagnation and blood stasis, phlegm and blood stasis syndromes, improve the general state of rats. Conclusion: the treatment of phlegm and blood stasis can improve the syndromes of phlegm and blood stasis, reduce the chronic airway inflammation and adjust the immune abnormality in CVA model rats.
【作者单位】：辽宁中医药大学附属医院儿科;沈阳市皇姑区中医院;
【基金】：沈阳市科学技术研究项目(F14-158-9-09)
【分类号】：R285.5;R-332

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