窒息法与高钾合并窒息法制备心肺复苏后全身炎症反应小鼠模型的比较研究

发布时间：2018-07-12 18:27

本文选题：心肺复苏 + 全身炎症反应　；参考：《临床急诊杂志》2016年01期

【摘要】：目的:探讨C57BL/6小鼠心搏骤停心肺复苏标准化动物模型,为研究心搏骤停后全身炎症反应综合征(SIRS)、多器官功能障碍综合征(MODS)建立实验室基础和必要条件。方法:随机选取80只C57BL/6小鼠应用窒息法制备心肺复苏模型(窒息组),65只C57BL/6小鼠应用高钾合并窒息法制备心肺复苏模型(高钾合并窒息组),将2组小鼠再随机分为复苏前组、复苏后2、12、24h组。观察各组小鼠自主循环恢复(ROSC)率和生存率;运用酶联免疫吸附法(ELISA)检测各组肺泡灌洗液IL-1β、IL-6、IL-10、IFN-γ浓度;比较复苏后心脏、肾脏、脑组织形态学改变以及全身炎症反应程度。结果:与窒息组比较,高钾合并窒息组ROSC率较低,复苏后2、12h小鼠存活率较低,复苏后24h小鼠存活率一致。与复苏前比较,2组模型在复苏后2、12、24h肺泡灌洗液中IL-1β、IL-6、IL-10、IFN-γ的浓度均显著上升。与窒息组比较,高钾合并窒息组在复苏后12h炎症反应表现更突出,2组在复苏后24h呈现下降趋势,但仍显著高于心肺复苏前的基线水平。与复苏前比较,2组模型在复苏后2、12、24h心肌组织和肾脏组织磷酸化IκB-α(p-IκB-α)蛋白表达显著上升,与窒息组复苏后同一时间点比较,高钾合并窒息组在复苏后2、12h肾脏组织p-IκB-α蛋白显著较高。结论:窒息模型是现今制备小鼠心肺复苏模型中较为常用的方法,不需要任何有创的外科手术,模型复苏成功率高,心脏、肾脏、脑组织形态学改变与高钾合并窒息组一致。高钾合并窒息小鼠心肺复苏模型,呼吸心跳骤停的起始时间可以达到完全一致,组织缺血缺氧损伤的时间更确切,全身炎症反应更显著,是值得推广应用的小鼠心搏骤停心肺复苏标准化动物模型。
[Abstract]:Objective: to study the standardized animal model of cardiopulmonary resuscitation (CPR) in C57BL / 6 mice and to establish the laboratory basis and necessary conditions for the study of systemic inflammatory response syndrome (Sirs) and multiple organ dysfunction syndrome (mods) after cardiac arrest. Methods: a total of 80 C57BL / 6 mice were randomly selected to establish cardiopulmonary resuscitation model (asphyxia group) by asphyxiating method. 65 C57BL / 6 mice were used to establish cardiopulmonary resuscitation model (hyperkalemia combined with asphyxia group) with hyperkalemia combined with asphyxia. The two groups were randomly divided into two groups before resuscitation. After resuscitation, 24 hours after resuscitation. The rate of spontaneous circulation recovery (ROSC) and survival rate of each group were observed, the concentration of IL-1 尾 and IL-6 IL-10 IFN- 纬 in alveolar lavage fluid of each group was detected by Elisa, and the changes of heart, kidney, brain tissue and systemic inflammatory reaction were compared after resuscitation. Results: compared with the asphyxia group, the ROSC rate of the hyperkalemia combined with asphyxia group was lower, the survival rate of the mice at 2h after resuscitation was lower, and the survival rate was the same at 24h after resuscitation. Compared with those before resuscitation, the concentration of IL-1 尾, IL-6, IL-10 and IFN- 纬 in the alveolar lavage fluid of the two groups increased significantly after resuscitation for 24 h after resuscitation. Compared with the asphyxia group, the inflammatory response in the hyperkalemia combined with asphyxia group was more prominent at 12 hours after resuscitation, but still significantly higher than the baseline level before cardiopulmonary resuscitation. Compared with those before resuscitation, the expression of phosphorylated I 魏 B- 伪 (p-I 魏 B- 伪) protein in myocardial and renal tissues was significantly increased at 212h after resuscitation. Compared with that in asphyxia group at the same time point after resuscitation, the expression of p-I 魏 B- 伪 protein in renal tissue was significantly higher in the hyperkalemia combined with asphyxia group at 212h after resuscitation. Conclusion: asphyxia model is a common method in the preparation of cardiopulmonary resuscitation (CPR) model in mice. There is no need for any invasive surgical operation. The success rate of resuscitation is high, and the morphologic changes of heart, kidney and brain tissue are consistent with those of hyperkalemia combined with asphyxia group. In the cardiopulmonary resuscitation model of mice with hyperkalemia and asphyxia, the onset time of respiratory and cardiac arrest was completely consistent, the time of tissue ischemia and hypoxia injury was more accurate, and the systemic inflammatory reaction was more significant. It is a standardized animal model of cardiac arrest and cardiopulmonary resuscitation in mice.
【作者单位】：武汉大学人民医院急诊科;
【基金】：国家自然科学基金(No:81372020) 武汉市中青年医学骨干人才培养项目(No:2014ZX0001)
【分类号】：R459.7;R-332

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