黄芩苷对注意缺陷多动障碍模型大鼠行为学特征的影响研究

发布时间：2018-07-22 14:31

【摘要】：目的研究黄芩苷对注意缺陷多动障碍(ADHD)模型大鼠行为学特征的影响,为黄芩苷治疗ADHD的进一步研究提供依据。方法将40只SHR大鼠随机分为模型组、盐酸哌甲酯组及黄芩苷低、中、高剂量组,每组8只,另设8只WKY大鼠为正常对照组。盐酸哌甲酯组(0.07 mg/mL)及黄芩苷低剂量(3.33 mg/mL)、中剂量(6.67 mg/mL)、高剂量(10 mg/mL)组分别按体重(1.5 mL/100 g)给予对应药物灌胃,正常对照组、模型组给予等量生理盐水灌胃,各组大鼠灌胃4周,每日2次。旷场实验观察实验第0天及灌胃后7、14、21、28 d各组大鼠的总运动距离及平均运动速度,评估药物对大鼠多动、冲动行为的控制作用;Morris水迷宫实验观察各组大鼠的潜伏期、目标象限活动比率及穿越平台次数,评估药物对大鼠注意力的影响。结果旷场实验数据显示,第0天,模型组及各用药组大鼠的总运动距离及平均运动速度均显著高于正常对照组(P0.05);第7天,盐酸哌甲酯组总运动距离及平均运动速度较模型组显著降低(P0.05);第14天,盐酸哌甲酯组及黄芩苷高剂量组大鼠在总运动距离及平均运动速度上均较模型组显著降低(P0.05);第21天及第28天,黄芩苷各剂量组在总运动距离及平均运动速度上较模型组均呈不断下降趋势(P0.05)。水迷宫空间探索实验结果显示,盐酸哌甲酯组及黄芩苷中、高剂量组目标象限停留时间比率显著高于模型组(P0.05),盐酸哌甲酯组、黄芩苷高剂量组目标象限运动距离所占比率亦显著高于模型组(P0.05);黄芩苷高剂量组穿越平台次数高于其余各组(P0.05)。结论黄芩苷及盐酸哌甲酯均能够调控ADHD模型SHR大鼠的运动能力及学习记忆能力,从而控制ADHD多动、冲动及注意力不集中的核心症状;黄芩苷药效呈剂量依赖性,高剂量黄芩苷疗效最为显著,但其药效的发挥迟于盐酸哌甲酯。
[Abstract]:Objective to study the effect of Baicalin on the behavioral characteristics of the model rats with attention deficit hyperactivity disorder (ADHD), and to provide a basis for further study of Baicalin in the treatment of ADHD. Methods 40 SHR rats were randomly divided into model group, methylphenidate group and Baicalin in low, middle, high dose group, 8 rats in each group, and 8 WKY rats were normal control group. The methyl group (0.07 mg/mL) and baicalin were low dose (3.33 mg/mL), middle dose (6.67 mg/mL), and high dose (10 mg/mL) group were given the corresponding medicine according to body weight (1.5 mL/100 g), and the normal control group was given the normal control group. The model group was given the same amount of normal saline for 4 weeks, 2 times a day. The open field experiment was observed for zeroth days and 7,14,21,28 d after gavage. The total movement distance and average speed of the rats in each group were used to evaluate the effect of drugs on the hyperactivity and impulse behavior of rats. The Morris water maze test observed the latency of the rats, the ratio of target quadrants and the number of crossing platforms, and evaluated the effect of the drug on the attention of the rats. The results of the open field experiment showed that the model group and the model group were zeroth days. The total movement distance and average motor speed of the rats in each group were significantly higher than that of the normal control group (P0.05). The total movement distance and average velocity of the methyl piperidine hydrochloride group were significantly lower than those of the model group (P0.05) on the seventh day. The fourteenth day, the total movement distance and the average movement speed of the high dose group of methylphenidate and baicalin were compared with the model rats. In the twenty-first and 28 days, the total movement distance and average velocity of Baicalin in the twenty-first days and the 28 days were lower than those in the model group (P0.05). The results of the water maze space exploration experiment showed that the ratio of the target residence time of the high dose group was significantly higher than that of the model group (P0.05). The ratio of target quadrant distance of high dose baicalin group was significantly higher than that of model group (P0.05), and the number of high dose group of baicalin high dose group was higher than that of other groups (P0.05). Conclusion baicalin and methylphenidate can control the exercise ability and learning and memory ability of ADHD model SHR rats, thus controlling ADHD hyperactivity. The core symptoms of impulsiveness and concentration were not concentrated; the efficacy of baicalin was dose-dependent and the effect of high dose baicalin was most significant, but its efficacy was later than methylphenidate.
【作者单位】：南京中医药大学中医儿科研究所/江苏省儿童呼吸疾病(中医药)重点实验室;
【基金】：江苏省教育厅研究生创新工程项目(KYZZ16_0410) 国家自然科学基金(81273801)
【分类号】：R285.5;R-332

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