苓桂术甘汤调节心室重构模型大鼠心肌组织NF-κB信号通路的分子机制研究

发布时间：2018-07-24 07:48

【摘要】：目的:观察苓桂术甘汤对心室重构大鼠心肌组织NF-κB信号通路相关分子表达的影响,探讨其抑制心室重构的分子机制。方法:采用冠脉结扎复制心梗后心室重构大鼠模型,造模2 w后,药物连续干预4 w,采用Western blot法检测模型大鼠心肌组织NF-κBp65、IKK-β、IκB-α及p-IκBα的蛋白表达,采用ELISA法检测大鼠血清中TNF-α、IL-1β及IL-6的含量,采用HE及Masson染色观察大鼠心肌组织病理学变化。结果:心室重构模型大鼠出现明显的心肌细胞损伤及间质纤维化等病理变化,与假手术组比较,心肌组织NF-κBp65、p-IκBα蛋白表达显著升高,IKK-β、IκB-α蛋白表达显著降低,血清TNF-α、IL-1β、IL-6含量显著升高(P0.01)。与模型组比较,苓桂术甘汤各剂量干预4 w后,可显著改善模型大鼠心肌组织损伤,抑制NF-κBp65、p-IκBα蛋白表达、上调IKK-β、IκB-α蛋白表达,降低血清TNF-α、IL-1β及IL-6含量(P0.05或P0.01)。结论:苓桂术甘汤改善心肌组织损伤、抵制心室重构的作用机制与抑制心肌组织NF-κB信号通路过度激活有关。
[Abstract]:Objective: To observe the effect of Ling Gui Shu Gan Tang on the expression of NF- kappa B signal pathway related molecules in cardiac muscle tissue of rats with ventricular remodeling and to explore the molecular mechanism of inhibiting ventricular remodeling. Methods: the rat model of ventricular remodeling after coronary artery ligature was ligated and replicated after myocardial infarction. After 2 W, the drug was continuously dried to 4 W, and Western blot method was used to detect the myocardium of the model rats. The protein expression of NF- kappa Bp65, IKK- beta, I kappa B- A and p-I kappa B alpha was expressed. The content of TNF- alpha, IL-1 beta and IL-6 in rat serum was detected by ELISA method. The pathological changes of myocardium in rats were observed by HE and staining. Results: the pathological changes of cardiac muscle injury and interstitial fibrosis were observed in the rat model of ventricular remodeling, compared with the sham operation group. In comparison, the expression of NF- kappa Bp65 and p-I kappa B alpha was significantly increased, the expression of IKK- beta, I kappa B- alpha was significantly reduced, the content of serum TNF- a, IL-1 beta and IL-6 increased significantly (P0.01). Compared with the model group, the myocardial injury of the model rats could be significantly improved after the intervention of 4 W. The expression of I - kappa B- alpha protein and the decrease of serum TNF- alpha, IL-1 beta and IL-6 content (P0.05 or P0.01). Conclusion: Luli Gump soup improves myocardial injury, and the mechanism of resisting ventricular remodeling is related to the inhibition of the excessive activation of NF- kappa B signal pathway in myocardial tissue.
【作者单位】：安徽中医药大学;安徽省中医药科学院中西医结合研究所;
【基金】：国家自然科学基金面上项目(30973707,81373533);国家自然科学基金青年项目(81202631) 安徽省自然科学基金青年项目(1508085QH192,1608085QH222) 安徽省教育厅优秀青年人才支持重点项目(gxyq ZD2016131)
【分类号】：R285.5;R-332

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