黄芪百合颗粒对高原低氧模型小鼠脑氧化应激损伤的影响
发布时间：2018-07-24 19:51
【摘要】：目的探讨黄芪百合颗粒对高原低氧模型小鼠脑组织氧化应激损伤的影响。方法将60只SPF级昆明小鼠随机分为对照组、模型组、黄芪百合颗粒低、中、高(1.75、3.5、7 mg·kg~(-1))剂量组。常规饲养3 d后,灌胃给药,每日1次,连续17 d。从第14日起每日灌胃30 min后除对照组外其余各组小鼠于低氧舱中模拟高海拔低压低氧环境进行暴露,持续暴露3 d,眼球采血后处死小鼠。采用比色分析法测定脑组织中超氧化物歧化酶活性、丙二醛含量及总抗氧化能力,HE染色在显微镜下观察脑组织的形态变化进行病理评分并计数炎细胞数量。结果与对照组比较,模型组小鼠脑质量明显增加,超氧化物歧化酶活性和总抗氧化能力明显降低,脑丙二醛含量、脑组织病理评分和炎细胞数量均明显增加(P0.01)。与模型组比较,黄芪百合颗粒中、高剂量组小鼠的脑组织超氧化物歧化酶活性和总抗氧化能力均升高,丙二醛含量、病理评分及炎细胞数均降低(P0.05或P0.01)。结论黄芪百合颗粒对高原低压低氧环境下小鼠脑损伤具有良好保护作用,可能是通过增强脑组织自由基清除能力实现的。
[Abstract]:Objective to investigate the effect of Huangqi lily granule on oxidative stress injury in brain tissue of high altitude hypoxia model mice. Methods Sixty Kunming mice of SPF grade were randomly divided into control group, model group, low, medium and high dose group (1.75 渭 g / kg ~ (-1). After 3 days of routine feeding, the drug was administered by gavage once a day for 17 days. The mice in each group were exposed to hypoxia-hypoxia environment at high altitude and low pressure and hypoxia for 30 min from the 14th day, and the mice were killed after eyeball blood collection for 3 days. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the total antioxidant capacity of brain tissue were determined by colorimetric analysis. The morphological changes of brain tissue were observed under microscope and the number of inflammatory cells was counted. Results compared with the control group, the brain mass of the model group was significantly increased, the activity of superoxide dismutase (SOD) and the total antioxidant capacity were significantly decreased, the content of malondialdehyde (MDA) in the brain, the pathological score of brain tissue and the number of inflammatory cells were significantly increased (P0.01). Compared with the model group, the activity of superoxide dismutase (SOD) and the total antioxidant ability of the mice in high dose group were increased, the content of malondialdehyde (MDA), pathological score and the number of inflammatory cells were decreased (P0.05 or P0.01). Conclusion Astragalus lily granule has a good protective effect on brain injury in mice under high altitude low pressure and hypoxia, which may be achieved by enhancing the free radical scavenging ability of brain tissue.
【作者单位】： 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室;敦煌医学与转化省部共建教育部重点实验室;
【基金】：2012甘肃省科技支撑计划项目,No.120FKCA169 陇原青年创新人才扶持计划,No.2014-93-72
【分类号】：R285.5;R-332
，
本文编号：2142474
[Abstract]:Objective to investigate the effect of Huangqi lily granule on oxidative stress injury in brain tissue of high altitude hypoxia model mice. Methods Sixty Kunming mice of SPF grade were randomly divided into control group, model group, low, medium and high dose group (1.75 渭 g / kg ~ (-1). After 3 days of routine feeding, the drug was administered by gavage once a day for 17 days. The mice in each group were exposed to hypoxia-hypoxia environment at high altitude and low pressure and hypoxia for 30 min from the 14th day, and the mice were killed after eyeball blood collection for 3 days. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the total antioxidant capacity of brain tissue were determined by colorimetric analysis. The morphological changes of brain tissue were observed under microscope and the number of inflammatory cells was counted. Results compared with the control group, the brain mass of the model group was significantly increased, the activity of superoxide dismutase (SOD) and the total antioxidant capacity were significantly decreased, the content of malondialdehyde (MDA) in the brain, the pathological score of brain tissue and the number of inflammatory cells were significantly increased (P0.01). Compared with the model group, the activity of superoxide dismutase (SOD) and the total antioxidant ability of the mice in high dose group were increased, the content of malondialdehyde (MDA), pathological score and the number of inflammatory cells were decreased (P0.05 or P0.01). Conclusion Astragalus lily granule has a good protective effect on brain injury in mice under high altitude low pressure and hypoxia, which may be achieved by enhancing the free radical scavenging ability of brain tissue.
【作者单位】： 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室;敦煌医学与转化省部共建教育部重点实验室;
【基金】：2012甘肃省科技支撑计划项目,No.120FKCA169 陇原青年创新人才扶持计划,No.2014-93-72
【分类号】：R285.5;R-332
，
本文编号：2142474
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