益生菌制剂对抗生素诱导腹泻模型小鼠肠道菌群的恢复

发布时间：2018-07-27 13:57

【摘要】：为了评价益生菌制剂对腹泻模型小鼠肠道菌群的恢复,现采用22.4 g/(kg·d)氨苄青霉素灌胃构造小鼠腹泻模型,其中空白对照组灌胃等量生理盐水。造模成功后,用低、中、高不同剂量的益生菌制剂对小鼠治疗恢复,治疗14 d后,观察益生菌制剂对小鼠稀便率、稀便级和腹泻指数的影响。无菌取治疗后0、7、14 d的小鼠粪便进行分析,分别采用平板计数和DGGE技术检测肠道目标菌群变化。停用益生菌制剂后,无菌采取0、7、14 d的粪便样品,荧光定量PCR定量检测外源益生菌。结果表明,益生菌制剂不仅能改善小鼠的腹泻情况,促进腹泻小鼠的生长,还对腹泻小鼠肠道菌群结构具有显著恢复作用;荧光定量PCR检测结果表明外源益生菌嗜酸乳杆菌NCFM、乳双歧杆菌Bi-07和鼠李糖乳杆菌HN001在停止灌胃益生菌制剂后可定殖存活,但随着时间推移呈一定衰减的趋势。
[Abstract]:In order to evaluate the effect of probiotics on the recovery of intestinal flora in mice with diarrhea, the diarrhea model was established by gastric administration of 22.4 g / (kg d) ampicillin, in which the blank control group was given the same amount of normal saline. After successful modeling, the mice were treated with low, medium and high doses of probiotics. After 14 days of treatment, the effects of probiotics on dilute stool rate, dilute stool grade and diarrhea index were observed. The faeces of mice treated with aseptic therapy for 14 days were analyzed. Plate count and DGGE technique were used to detect the changes of intestinal flora. After the probiotics were stopped, the fecal samples of 0 ~ 7 ~ 14 days were used for quantitative detection of exogenous probiotics by fluorescence quantitative PCR. The results showed that probiotics could not only improve the diarrhea of mice and promote the growth of diarrhea mice, but also significantly restore the intestinal flora structure of diarrhea mice. Fluorescence quantitative PCR analysis showed that exogenous probiotics, Lactobacillus acidophilus NCFM, Lactobacillus acidophilus Bi-07 and Lactobacillus rhamnoides HN001 could colonize and survive after stopping gastric administration of probiotics, but tended to decrease with time.
【作者单位】：无限极(中国)有限公司;上海交通大学农业与生物学院;华南理工大学食品科学与工程学院;
【基金】：广东省自然科学基金项目(2014A030313242) 中央高校科研业务费项目(2015ZZ110)
【分类号】：R-332;TS201.3

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