腺病毒7d基因型与7b基因型全基因组比较分析
发布时间：2018-07-27 11:53
【摘要】：为了解重庆地区流行的7型腺病毒(Human adenovirus 7,HAdV-7)基因型型别,从全基因组水平探讨HAdV-7b和HAdV-7d可能的致病性差异,收集儿童急性下呼吸道感染患儿的呼吸道分泌物标本分离培养,提取DNA后进行HAdV-7基因型别鉴定、限制性酶切分析和全基因组测序,再利用MEGA6比较分析。本研究在2009年6月~2013年1月间共收集4334份急性下呼吸道感染患儿的鼻咽抽吸物标本,随机选取2411份进行病毒分离培养,共有164份(164/2411,6.8%)鼻咽抽吸物HAdV阳性,其中HAdV-7 87例。限制性酶切分析显示,随机挑选的3株HAdV-7分离株均为HAdV-7d。CQ1198株行全基因组测序并与其他HAdV-7全基因组构建进化树,结果发现16株中国地区的HAdV-7为HAdV-7d基因型,且上述16株HAdV-7与7d2的同源性为99.9%,与7b的同源性为98.2%。相较于ak40株(HAdV-7b),CQ1198共有49处核苷酸替换,其中20处可导致非同义替换;4处核苷酸插入,其中24bp和18bp 2个片段插入分别位于病毒相关RNAⅡ(Virus-associated RNAⅡ,VA RNAⅡ)和L3的pⅥ区域。HAdV-7可有多个基因型致病,且一直处于进化过程中,其主要流行基因型由HAdV-7b变为HAdV-7d。应加强对HAdV分子流行病学监测,明确其进化规律,为HAdV疫苗研发提供分子流行病学依据。
[Abstract]:In order to understand the genotypes of adenovirus type 7 (Human adenovirus 7) HAdV-7, the possible pathogenicity differences between HAdV-7b and HAdV-7d were studied at the whole genome level. The samples of respiratory secretions from children with acute lower respiratory infection were collected and cultured. HAdV-7 genotyping, restriction enzyme digestion and whole genome sequencing were performed after DNA extraction, and then MEGA6 was used to compare and analyze. From June 2009 to January 2013, a total of 4334 nasopharyngeal aspiration samples were collected from children with acute lower respiratory tract infection. 2411 samples were isolated and cultured randomly. A total of 164 cases (164 / 24116.8%) were HAdV positive in nasopharynx aspiration, among which 87 cases were HAdV-7. Restriction endonuclease analysis showed that all of the three randomly selected HAdV-7 isolates were HAdV-7d.CQ1198 strains and constructed phylogenetic tree with other HAdV-7 genomes. The results showed that 16 HAdV-7 strains in China were HAdV-7d genotypes. The homology between HAdV-7 and 7d2 was 99.9 and 98.2 with 7b. Compared with ak40 strain (HAdV-7b), there were 49 nucleotide substitutions in CQ1198, 20 of which resulted in 4 nucleotide insertions in non-synonymous substitutions. The 24bp and 18bp fragments were inserted into the p鈪，
本文编号：2147741
[Abstract]:In order to understand the genotypes of adenovirus type 7 (Human adenovirus 7) HAdV-7, the possible pathogenicity differences between HAdV-7b and HAdV-7d were studied at the whole genome level. The samples of respiratory secretions from children with acute lower respiratory infection were collected and cultured. HAdV-7 genotyping, restriction enzyme digestion and whole genome sequencing were performed after DNA extraction, and then MEGA6 was used to compare and analyze. From June 2009 to January 2013, a total of 4334 nasopharyngeal aspiration samples were collected from children with acute lower respiratory tract infection. 2411 samples were isolated and cultured randomly. A total of 164 cases (164 / 24116.8%) were HAdV positive in nasopharynx aspiration, among which 87 cases were HAdV-7. Restriction endonuclease analysis showed that all of the three randomly selected HAdV-7 isolates were HAdV-7d.CQ1198 strains and constructed phylogenetic tree with other HAdV-7 genomes. The results showed that 16 HAdV-7 strains in China were HAdV-7d genotypes. The homology between HAdV-7 and 7d2 was 99.9 and 98.2 with 7b. Compared with ak40 strain (HAdV-7b), there were 49 nucleotide substitutions in CQ1198, 20 of which resulted in 4 nucleotide insertions in non-synonymous substitutions. The 24bp and 18bp fragments were inserted into the p鈪，
本文编号：2147741
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