大鼠肺间质纤维化造模的2种方法比较研究

发布时间：2018-07-28 07:40

【摘要】：目的探讨气管和腹腔注射2种给药方式复制特发性肺纤维化(idiopathic pulmonary fibrosis,IPF)大鼠模型的差异。方法240~260g雄性SD大鼠24只,按照随机数字表法分为气管对照组(QC组)、气管给药组(Q组)、腹腔对照组(FC组)和腹腔给药组(F组),每组6只。分别经气管一次性注射生理盐水0.2mL或博莱霉素(BLM)5mg·kg~(-1)·d~(-1);腹腔注射生理盐水0.2mL或博莱霉素15mg·kg~(-1)·d~(-1),连续10d。28d后处死。观察每组小鼠生存率、体质量、肺脏外观、病理改变及肺组织羟脯氨酸含量。结果 Q组生存率为66.7%;F组生存率为83.3%,二者比较差异有统计学意义(P0.05)。2种方法给药组大鼠体质量均减轻,F组动物体质量在1~7d内下降,第8天起开始恢复;Q组动物体质量在前15d内持续下降,第16d起开始恢复。2种方法给药组的肺脏外观基本相同,没有显著差别。病理检查证实,Q组的胶原纤维沉积主要分布在气管周围,而F组的胶原沉积则分布在胸膜和肺间质。2种方法复制的模型组肺组织羟脯氨酸含量均显著高于对照组,差异有统计学意义(P0.05);模型组间差异无统计学意义。结论气管给药与腹腔给药对于复制大鼠IPF无明显差别,但气管给药法更方便、经济和可靠。
[Abstract]:Objective to investigate the difference between trachea and intraperitoneal administration of (idiopathic pulmonary fibrosis (IPF) rat models of idiopathic pulmonary fibrosis (IPF). Methods 24 male SD rats were randomly divided into three groups: trachea control group (QC group), tracheal administration group (Q group), peritoneal control group (FC group) and intraperitoneal administration group (F group), with 6 rats in each group. Normal saline 0.2mL or bleomycin (BLM) 5mg kg ~ (-1) d ~ (-1) were injected through trachea and intraperitoneal injection of normal saline 0.2mL or bleomycin 15mg (15mg ~ (-1) d ~ (-1) were performed after 10d.28d. Survival rate, body mass, lung appearance, pathological changes and hydroxyproline content were observed in each group. Results the survival rate of group Q was 66.7% and the survival rate of group F was 83.3%. The difference between the two groups was statistically significant (P0.05). On the 8th day, the mass of animals and animals in group Q decreased continuously in the first 15 days, and the lung appearance of group 2 was the same as that of the control group on the 16th day, and there was no significant difference between the two groups. Pathological examination showed that collagen fiber deposition in group Q was mainly distributed around trachea, while collagen deposition in group F was significantly higher than that in control group (P < 0.01), but the content of hydroxyproline in lung tissue of group F was significantly higher than that of control group (P < 0.01), while that of group F was significantly higher than that of control group (P < 0.05). The difference was statistically significant (P0.05); there was no significant difference between the model groups. Conclusion there is no significant difference between tracheal administration and intraperitoneal administration in replicating IPF in rats, but tracheal administration is more convenient, economical and reliable.
【作者单位】：第四军医大学药学院天然药物学教研室;第四军医大学学员旅;
【分类号】：R563;R-332

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