真武汤对2型心肾综合征模型大鼠心脏和肾脏的保护作用

发布时间：2018-07-28 13:33

【摘要】：目的:探讨真武汤对2型心肾综合征大鼠心脏和肾脏的保护作用。方法:通过结扎左冠状动脉前降支联合肾大部分切除手术构建2型心肾综合征大鼠模型。32只SD大鼠随机分成真武汤低、中、高浓度干预组和对照组。干预组分别给予不同生药量的真武汤灌胃,对照组给予等剂量蒸馏水灌胃。干预结束后比较大鼠的生化指标、心脏超声检测和免疫组化结果。结果:高浓度组血尿素氮、血肌酐较对照组明显降低,尿硫酸吲哚酚较对照组明显升高,且高浓度组血尿素氮较低浓度组明显降低(P0.05)。中浓度组血尿素氮、血肌酐较对照组明显降低(P0.05)。低、中、高浓度组左室后壁(收缩期)、心排量、每搏输出量较对照组明显增加(P0.05)。另外高浓度组较对照组左室内径(舒张)和左室容积(收缩)明显增加(P0.05)。Masson染色、CollagenⅠ免疫荧光染色、TGF-β免疫荧光染色均表明低、中、高浓度组的心脏和肾脏组织纤维化较对照组减轻。结论:真武汤对2型心肾综合征大鼠心脏和肾脏具有一定的保护作用。该作用可能通过增加尿毒素排出和减轻组织纤维化来实现。
[Abstract]:Objective: To explore the protective effect of Zhenwu Decoction on heart and kidney of type 2 heart kidney syndrome in rats. Methods: by ligating the anterior descending branch of the left coronary artery and the most nephrectomy of the kidney, the rat model of type 2.32 rats was randomly divided into the low, middle, high concentration dry preconditioning group and the control group. The intervention group was given different drugs. After the intervention, the biochemical indexes of the rats, the echocardiography and the immunohistochemical results were compared. Results: the blood urea nitrogen and the serum creatinine in the high concentration group were significantly lower than those in the control group, and the urine indolol sulfate was significantly higher than the control group, and the low concentration group of blood urea nitrogen in the high concentration group was significantly lower than that of the control group. The blood urea nitrogen and blood creatinine in the medium concentration group were significantly lower than those in the control group (P0.05). The left ventricular posterior wall (systole) and cardiac output were significantly increased (P0.05) in the low, middle and high concentration group, and the left ventricular diameter (diastolic) and left ventricular volume (P0.05) in the high concentration group were significantly increased (P0.05).Masson staining (P0.05), Collagen I immunofluorescence staining, TGF- beta immunofluorescence staining showed that the heart and renal tissue fibrosis in the middle and high concentration groups were lower than those in the control group. Conclusion: Zhenwu decoction has a certain protective effect on the heart and kidney of type 2 heart kidney syndrome rats. This effect may be achieved by increasing the excretion of urein and reducing tissue fibrosis.
【作者单位】： 南方医科大学珠江医院心血管内科;
【基金】：国家自然科学基金面上项目(81573732) 广东省自然科学基金项目(2015A030313304)
【分类号】：R285.5;R-332
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本文编号：2150311