金丝桃苷抑制肺炎支原体肺炎模型小鼠的损伤

发布时间：2018-07-28 17:21

【摘要】：目的:探讨金丝桃苷对肺炎支原体肺炎(MPP)小鼠的保护作用及其调控机制。方法:选取BALB/c小鼠40只,随机分为对照组、模型组、金丝桃苷低剂量(12.5 mg/kg)组和金丝桃苷高剂量(50 mg/kg)组。以肺炎支原体国际标准株(MPFH)滴鼻造模,第2天开始治疗,治疗3 d后进行后续实验。HE染色检测肺组织病理改变;全自动生化分析仪检测血清中C-反应蛋白(CRP)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)和心肌型肌酸激酶同工酶(CK-MB)水平,检测肺组织中活性氧簇(ROS)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)水平;ELISA和Western blot方法分别检测血清和肺组织中白细胞介素1β(IL-1β)、IL-6、IL-18和肿瘤坏死因子α(TNF-α)水平;RT-q PCR和Western blot方法检测NLRP3、ASC和caspase-1的mRNA和蛋白表达。结果:与对照组相比,模型组小鼠的肺组织中出现炎症反应、肺泡间质增宽等病理变化;CRP、ALT、AST、LDH和CK-MB水平显著上调(P0.05);ROS水平显著上升,而SOD和GSH水平显著下降(P0.05);IL-1β、IL-6、IL-18和TNF-α水平显著上调(P0.05);NLRP3、ASC和caspase-1的蛋白表达也显著升高(P0.05)。与模型组相比,金丝桃苷组中的上述症状明显减轻,且高剂量比低剂量组的缓解效果更明显。结论:金丝桃苷能够抑制MPP小鼠的损伤,这可能与其抑制氧化应激和NLRP3炎性体的激活有关。
[Abstract]:Objective: to investigate the protective effect of hyperin on mycoplasma pneumoniae pneumonia (MPP) mice and its regulatory mechanism. Methods: forty BALB/c mice were randomly divided into control group, model group, low dose hyperoside (12.5 mg/kg) group and hypericin high dose (50 mg/kg) group. The mycoplasma pneumoniae (MPFH) strain was used as nasal drip model. After 3 days of treatment, the pathological changes of lung tissue were detected by HE staining. Serum C-reactive protein (CRP),) alanine aminotransferase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) and myocardial creatine kinase isoenzyme (CK-MB) were detected by automatic biochemical analyzer. Detection of reactive oxygen species (ROS), superoxide dismutase (SOD) and glutathione (GSH) levels in lung tissues Elisa and Western blot were used to detect interleukin-1 尾 (IL-1 尾) IL-6 IL-18 and tumor necrosis factor- 伪 (TNF- 伪) in serum and lung tissues respectively. RT-q PCR and Western blot methods were used to detect NLRP3ASC. And the expression of mRNA and protein in caspase-1. Results: compared with the control group, inflammatory reaction was found in the lung tissue of the model group, and the levels of LDH and CK-MB were significantly up-regulated (P0.05) in the model group. The levels of SOD and GSH decreased significantly (P0.05). The levels of IL-18 and TNF- 伪 increased significantly (P0.05) and the protein expression of NLRP3ASC and caspase-1 increased significantly (P0.05). Compared with the model group, hyperoside group was more effective than the low dose group. Conclusion: hyperin can inhibit the damage of MPP mice, which may be related to the inhibition of oxidative stress and the activation of NLRP3 inflammatory body.
【作者单位】：河南省中医院儿科;
【分类号】：R285.5;R-332

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