腺苷A2A受体拮抗剂对大鼠氯化锂-毛果芸香碱所致癫痫模型的影响

发布时间：2018-07-31 10:10

【摘要】：目的研究腺苷A2A受体阻断剂对大鼠氯化锂-毛果芸香碱癫痫持续状态(SE)模型的影响。方法选取50只WD大鼠随机分为对照组、模型组及A2A受体阻断剂组。模型组采用氯化锂-毛果芸香碱腹腔注射复制癫痫模型,A2 A受体阻断剂组在氯化锂-毛果芸香碱注射前15 min予腹腔给药(SCH58261 0.05 mg/kg),对照组给予同等剂量生理盐水。在成功诱导癫痫发作40 min后予地西泮及水合氯醛终止发作,并于发作终止后24 h留取标本。尼氏染色法检测三组中海马神经元损伤情况,Westernblot法检测MAPKs(JNK/p-JNK、P38/p-P38和ERK/p-ERK)表达变化。结果对照组、模型组及A2A受体阻断剂组双侧海马CA3区正常形态神经元计数分别为158.6±8.4、59.8±7.4和123.4±5.0,模型组神经元计数显著低于对照组,差异具有统计学意义(P0.05),A2A受体阻断剂组神经元计数显著高于模型组,差异具有统计学意义(P0.05)。Westernblot法检测显示p-JNK、p-P38和p-ERK在模型组中表达明显增多,在A2A受体阻断剂组中p-JNK和p-P38表达减少。结论氯化锂-毛果芸香碱模型中,腺苷A2A受体阻断剂可能通过抑制p-JNK他p-P38的表达对神经元损伤起到保护作用。
[Abstract]:Aim to study the effect of adenosine A 2A receptor blocker on (SE) model of lithium-pilocarpine epileptic status in rats. Methods 50 WD rats were randomly divided into control group, model group and A 2A receptor blocker group. The model group was treated with lithium-pilocarpine intraperitoneal injection and the control group was given intraperitoneal administration of lithium-pilocarpine (SCH58261 0.05 mg/kg) 15 min before the injection of lithium-pilocarpine, and the control group was given the same dose of normal saline. Diazepam and chloral hydrate were given to the epileptic seizure for 40 min and the specimens were collected 24 hours after the seizure was successfully induced. The expression of MAPKs (JNKP / p-JNKP38 / p-P38 and ERK/p-ERK) was detected by Western blot in the three groups. Results the number of normal neurons in the bilateral hippocampal CA3 region in the control group, model group and A2A receptor blocker group was 158.6 卤8.4 卤59.8 卤7.4 and 123.4 卤5.0, respectively. The number of neurons in the model group was significantly lower than that in the control group. The difference was statistically significant (P0.05) the number of neurons in the A2A receptor blocker group was significantly higher than that in the model group. The difference was statistically significant (P0.05) .Western blot analysis showed that the expression of p-JNKN p-P38 and p-ERK in the model group was significantly increased, and the expression of p-JNK and p-P38 in the A2A receptor blocker group was decreased. Conclusion in lithium-pilocarpine model, adenosine A 2A receptor antagonist may play a protective role in neuronal injury by inhibiting the expression of p-JNK p-P38.
【作者单位】：广东省深圳市南山区人民医院神经内科;华中科技大学附属同济医院神经内科;
【基金】：国家自然科学基金青年基金项目(81201006) 深圳市南山区技术研发和创意设计项目分项基金(南科研卫2012002;南科研卫2014015)
【分类号】：R-332;R742.1

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