单核增生性李斯特菌hly缺失菌株的构建及生物特性的初步鉴定
发布时间：2018-07-31 10:20
【摘要】：为研究单核增生性李斯特菌(Listeria monocytogenes,Lm)hly基因对毒力的影响,利用同源重组原理,使用穿梭载体,构建单核细胞增生性李斯特菌野生菌株EGD-e的hly基因缺失菌株EGD-eΔhly。细菌活性实验证明缺失菌株生长状态与亲本无差异,但EGD-eΔhly丧失溶血活性,细胞侵袭能力降低约90%,在10~7 cells/m L腹腔注射条件下不表现动物毒性。在转录水平上EGD-eΔhly的毒力基因inl C、prf A的表达量分别下降了81%和76%,act A、plc B的表达量分别提高了2.7倍和1.8倍。hly缺失菌株的成功构建及生物特性的初步研究结果为研究Lm致病机理提供依据。
[Abstract]:In order to study the effect of Listeria monocytogenes Lm (Lm) gene on virulence, using the homologous recombination principle and using shuttle vector, the hly gene deletion strain EGD-e 螖 hly. of Listeria monocytogenes EGD-e strain was constructed. The bacterial activity test showed that there was no difference between the growth state of the deficient strain and its parent, but EGD-e 螖 hly lost hemolytic activity, the cell invasion ability decreased about 90%, and no animal toxicity was observed under the condition of 10 7 cells/m L intraperitoneal injection. At the transcriptional level, the expression of virulence gene of EGD-e 螖 hly, inl CfA, decreased by 81% and 76%, respectively, and the expression of act-Aplc B increased by 2.7 times and 1.8 times, respectively, and the results of preliminary study on biological characteristics of Lm-induced strain were as follows: 1. 7 times and 1. 8 times respectively. The mechanism of disease provides basis.
【作者单位】： 上海理工大学医疗器械与食品学院;
【基金】：国家自然科学基金面上项目(31371776) 上海理工大学微创励志创新基金项目(YS30809101);上海理工大学研究生创新基金项目
【分类号】：R378
，
本文编号：2155287
[Abstract]:In order to study the effect of Listeria monocytogenes Lm (Lm) gene on virulence, using the homologous recombination principle and using shuttle vector, the hly gene deletion strain EGD-e 螖 hly. of Listeria monocytogenes EGD-e strain was constructed. The bacterial activity test showed that there was no difference between the growth state of the deficient strain and its parent, but EGD-e 螖 hly lost hemolytic activity, the cell invasion ability decreased about 90%, and no animal toxicity was observed under the condition of 10 7 cells/m L intraperitoneal injection. At the transcriptional level, the expression of virulence gene of EGD-e 螖 hly, inl CfA, decreased by 81% and 76%, respectively, and the expression of act-Aplc B increased by 2.7 times and 1.8 times, respectively, and the results of preliminary study on biological characteristics of Lm-induced strain were as follows: 1. 7 times and 1. 8 times respectively. The mechanism of disease provides basis.
【作者单位】： 上海理工大学医疗器械与食品学院;
【基金】：国家自然科学基金面上项目(31371776) 上海理工大学微创励志创新基金项目(YS30809101);上海理工大学研究生创新基金项目
【分类号】：R378
，
本文编号：2155287
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