hucMSCs源性外泌体对单核巨噬细胞生长及炎性因子IL-8、IL-12分泌水平的影响

发布时间：2018-08-07 20:21

【摘要】：目的初步探讨人脐带间充质干细胞(hucMSCs)来源的外泌体(exosome)对单核巨噬细胞生长及炎性因子IL-8、IL-12分泌的影响。方法将hucMSCs来源的exosome与单核巨噬细胞(THP-1细胞株)共培养,利用MTT法和Annexin V-FIFC/PI双染法检测共培养后THP-1细胞的增殖及凋亡情况;PCR法和ELISA法分别检测THP-1细胞炎性因子IL-8、IL-12的mRNA及蛋白的表达情况。结果 MTT及流式细胞术检测结果显示hucMSCs来源的exosome对THP-1细胞具有促进增殖和抑制凋亡的作用,PCR及ELISA方法结果显示,exosome能够降低THP-1细胞IL-8、IL-12 mRNA及蛋白的表达水平。结论 hucMSCs源性exosome能够影响单核巨噬细胞的生长,并降低炎性因子IL-8、IL-12的分泌水平,而这可能是微环境中间充质干细胞调控单核巨噬细胞的机制之一。
[Abstract]:Objective to investigate the effects of exocrine (exosome) derived from human umbilical cord mesenchymal stem cells (hucMSCs) on the growth of mononuclear macrophages and the secretion of interleukin-12 (IL-12) in human umbilical cord mesenchymal stem cells. Methods exosome derived from hucMSCs was co-cultured with mononuclear macrophage (THP-1 cell line). The proliferation and apoptosis of co-cultured THP-1 cells were detected by MTT and Annexin V-FIFC/PI double staining. The mRNA and protein expression of IL-8 IL-12 in THP-1 cells were detected by PCR and ELISA respectively. Results the results of MTT and flow cytometry showed that exosome derived from hucMSCs could promote proliferation and inhibit apoptosis of THP-1 cells. The results of ELISA and ELISA showed that exosome could decrease the expression of IL-8 and IL-12 mRNA and protein in THP-1 cells. Conclusion hucMSCs derived exosome can affect the growth of mononuclear macrophages and decrease the level of IL-8 IL-12, which may be one of the mechanisms of mesenchymal stem cells regulating mononuclear macrophages.
【作者单位】：安徽省蚌埠医学院临床检验诊断学实验中心;安徽省蚌埠医学院第一附属医院检验科;安徽省蚌埠医学院医学检验系;
【基金】：安徽省高校自然科学研究重点项目(No.KJ2016A466) 蚌埠医学院科研基金项目(No.BYKY1429ZD);蚌埠医学院研究生科研创新项目(No.Byycx1523)
【分类号】：R392

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