浙江省啮齿动物携带心病毒的遗传特征研究

发布时间：2018-08-14 16:46

【摘要】：目的了解我国浙江省的啮齿动物携带心病毒情况,并分析其遗传特征。方法用反转录-聚合酶链反应选啮齿动物粪便标本中的心病毒,扩增其全基因组;利用Lasergene软件分别对多聚蛋白、P1蛋白、2C+3CD蛋白序列与Gen Bank下载的序列进行同源性分析;分析其基因组与多聚蛋白的特征;利用Phy ML软件构建系统发生树。结果浙江省啮齿动物携带心病毒。毒株Ruian-Rn93-1、Longquan-Aa3-1、Ruian-Rn93-3的多聚蛋白、P1以及2C+3CD蛋白与心病毒B的同源性分别为71.9%~85.4%、65.2%~89.8%与78.3%~95.4%,属于心病毒B;毒株Wencheng-Rn416的多聚蛋白、P1以及2C+3CD蛋白与Boone心病毒的同源性分别为92.5%、85.3%与96.9%,属于心病毒C。系统发生分析也表明毒株Ruian-Rn93-1、Longquan-Aa3-1、Ruian-Rn93-3与心病毒B的亲缘关系最近,Wencheng-Rn416与Boone心病毒的亲缘关系最近。系统发育分析发现啮齿动物在心病毒的起源与进化过程中起着重要的作用,并且可能发生跨种间传播而感染人。结论我国浙江省啮齿动物携带心病毒,并可能跨种间传播到人。
[Abstract]:Objective to investigate the prevalence of heart virus in rodents in Zhejiang Province and to analyze its genetic characteristics. Methods the whole genome of cardiac virus was amplified by reverse transcription-polymerase chain reaction (RT-PCR) from fecal samples of rodents, and the sequence of P1-2C 3CD was analyzed by Lasergene software. The characteristics of genomes and polyproteins were analyzed, and the phylogenetic tree was constructed by Phy ML software. Results rodents in Zhejiang Province carried heart virus. The homology between Ruian-Rn93-1 and Ruian-Rn93-3 polyprotein P1 and 2C 3CD protein were 71.9% and 85.42%, respectively, and 78.3%, respectively. The homology of P1 protein and 2C 3CD protein of Wencheng-Rn416 was 92.5% and 996.63%, respectively. Phylogenetic analysis also showed that the relationship between Ruian-Rn93-1 (Ruian-Rn93-3) Ruian-Rn93-3 and heart virus B was the closest between Wencheng-Rn416 and Boone. Phylogenetic analysis revealed that rodents play an important role in the origin and evolution of cardiac viruses and may infect humans through interspecific transmission. Conclusion rodents in Zhejiang Province carry cardioviruses and may be transmitted to humans across species.
【作者单位】：中国疾病预防控制中心传染病预防控制所人兽共患病室传染病预防控制国家重点实验室;温州市疾病预防控制中心;龙泉市疾病预防控制中心;文成县疾病预防控制中心;瑞安市疾病预防控制中心;
【基金】：国家自然科学基金(No.81273014,81290343)~~
【分类号】：R373

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