正交法筛选组穴对脑缺血再灌注损伤模型大鼠细胞外信号调节激酶信号转导通路的影响

发布时间：2018-08-14 17:08

【摘要】：背景:既往电针治疗脑缺血再灌注损伤观察的针刺穴位搭配较少。目的:探讨电针正交实验所选组穴对脑缺血再灌注损伤后细胞外信号调节激酶信号通路的影响。方法:150只SD大鼠随机等分为5组,假手术组只暴露大脑中动脉;模型组及正交1,2,3号组建立脑缺血再灌注模型。正交1,2,3号组采用正交法筛选的穴位组合进行电针刺激。正交1号组为百会、曲池、足三里、尺泽,正交2号组为百会、足三里、尺泽、三阴交,正交3号组为合谷、足三里、尺泽、三阴交。结果与结论:与模型组相比,正交1,2,3号组大鼠神经功能缺损评分降低,脑梗死面积缩小,脑缺血区微血管密度数量及缺血侧皮质和海马CA1区中磷酸化细胞外信号调节激酶表达水平增加,且正交3号组变化最为显著。提示电针经正交法筛选的合谷、足三里、尺泽、三阴交能有效减少脑缺血再灌注大鼠的梗死面积,且该效应可能与其调节受损脑组织中细胞外信号调节激酶信号通路密切相关。
[Abstract]:Background: few acupuncture points have been observed in the treatment of cerebral ischemia reperfusion injury. Aim: to investigate the effect of acupoints selected by electroacupuncture orthogonal experiment on extracellular signal-regulated kinase signaling pathway after cerebral ischemia-reperfusion injury. Methods one hundred and fifty Sprague-Dawley rats were randomly divided into 5 groups: sham-operated group only exposed middle cerebral artery (MCAA), model group and orthogonal group 1, 2, 3 to establish cerebral ischemia-reperfusion model. The combination of acupoints selected by orthogonal method was used for electroacupuncture stimulation in group 2 and group 3. Orthogonal group 1 for Baihui, qu Chi, Zusanli, Zizawa, orthogonal Group 2 for Baihui, Zusanli, Zizawa, Sanyinjiao, orthogonal Group 3 for Hegu, Zusanli, Zizawa, Sanyinjiao. Results and conclusion: compared with the model group, the neurological deficit score and the infarct area of the rats in the orthogonal 1 / 2 and 3 groups were decreased. The number of microvessel density and the expression level of phosphorylated signal regulated kinase in the ischemic cortex and hippocampal CA1 region were increased, especially in the orthogonal group 3. The results suggest that electroacupuncture selected by orthogonal method can effectively reduce the infarct size of cerebral ischemia-reperfusion rats, and this effect may be closely related to the regulation of extracellular signal-regulated kinase signaling pathway in damaged brain tissue.
【作者单位】：广西中医药大学附属瑞康医院针灸科;南方医科大学;
【基金】：国家自然科学基金项目(81173355)~~
【分类号】：R245;R-332

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