温针灸对不同时间点KOA模型兔关节软骨中MMP-1、MMP-13表达的影响

发布时间：2018-08-19 17:34

【摘要】：目的:观察温针灸(warming moxibustion)对兔膝骨关节炎(knee osteoarthritis,KOA)软骨细胞中基质金属蛋白-1、13(matrix metalloproteinase-1、13,MMP-1、MMP-13)表达的影响,明确温针灸治疗KOA的作用机制。方法:选取70只新西兰大耳兔,其中10只作为空白对照组,剩余随机分为模型2周组、模型4周组、模型6周组、温针灸2周组、温针灸4周组、温针灸6周组。采用右后肢伸直位石膏固定制备KOA兔模型,模型2、4、6周组不予处理,温针灸2、4、6周组分别给予温针灸治疗2周,治疗结束后取材。采用免疫组化及RT-PCR检测各组治疗前后关节软骨中MMP-1、MMP-13蛋白及mRNA的表达。结果:与空白对照组比较,模型2周组、模型4周组、模型6周组软骨中MMP-1、MMP-13表达明显升高(P0.05),且各组间比较,差异有统计学意义(P0.05)。与相应模型组比较,温针灸治疗后,MMP-1、MMP-13的表达降低(P0.05),且各组间比较差异有统计学意义(P0.05)。结论:温针灸可降低KOA兔软骨中MMP-1、MMP-13的表达,说明温针灸治疗KOA可能与其抑制MMP-1、MMP-13的表达有关。
[Abstract]:Objective: to observe the effect of warming acupuncture and moxibustion (warming moxibustion) on the expression of matrix metalloproteinase-1 (MMP) -1MMP 13 (MMP-13) in chondrocytes of knee osteoarthritis (KOA), and to clarify the mechanism of warming acupuncture and moxibustion in the treatment of KOA. Methods: 70 New Zealand rabbits were randomly divided into two groups: model group (2 weeks), model group (4 weeks), model group (6 weeks), warm acupuncture group (2 weeks), warm acupuncture group (4 weeks) and warm acupuncture group (6 weeks). The KOA rabbit model was established by plaster fixation of right hind limb. The model was not treated in the 6-week group. The warm acupuncture group was treated with warm acupuncture for 2 weeks, and the material was taken after the treatment. Immunohistochemistry and RT-PCR were used to detect the expression of MMP-1 and MMP-13 and mRNA in articular cartilage before and after treatment. Results: compared with the blank control group, the expression of MMP-1 and MMP-13 in the cartilage of the model group at 2 weeks, the model group at 4 weeks and the model group at 6 weeks significantly increased (P0.05), and the differences among the groups were statistically significant (P0.05). Compared with the corresponding model group, the expression of MMP-1 and MMP-13 decreased after warming acupuncture treatment (P0.05), and the differences among the groups were statistically significant (P0.05). Conclusion: warm acupuncture can reduce the expression of MMP-1 and MMP-13 in the cartilage of KOA rabbits, which suggests that warm acupuncture treatment of KOA may be related to its inhibition of the expression of MMP-1 and MMP-13.
【作者单位】：宁夏医科大学总医院中医骨伤科;
【基金】：国家自然科学基金(81260545)
【分类号】：R245;R-332

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