FGL2在急性病毒感染过程中对T细胞免疫应答的影响研究

发布时间：2018-08-26 16:49

【摘要】：目的探讨纤维蛋白原样2凝血酶原酶(FGL2)在急性病毒感染过程中对T细胞的增殖、分化和功能的影响。方法模型一:淋巴细胞脉络丛脑膜炎病毒(LCMV)感染Fgl2基因敲除小鼠(实验组)与C57BL/6J对照小鼠,感染后第8天,检测T细胞的增殖、分化和功能。模型二:过继转移能够特异性识别LCMV的TCR转基因小鼠SMARTA或P14的T细胞到Fgl2基因敲除小鼠(实验组)和C57BL/6J对照小鼠内,LCMV感染后第8天,检测小鼠体内供体SMARTA或P14细胞的增殖和分化。结果FGL2在LCMV急性感染过程中表达上调(P0.05);实验组和对照组的滤泡辅助T细胞(T_(FH))、Th1细胞和效应CD8~+T细胞的频率和数量均无明显差异(P0.05);GC B细胞和浆细胞的数量和频率、LCMV特异性抗体的滴度以及效应CD8~+T细胞中表达TNF-α和CD107a/b的频率均无明显差异(P0.05)。结论 FGL2在急性病毒感染过程中不影响T细胞的免疫应答。
[Abstract]:Objective to investigate the effects of fibrinogen 2 prothrombin (FGL2) on the proliferation, differentiation and function of T cells during acute viral infection. Methods: model 1: Fgl2 gene knockout mice (experimental group) and C57BL/6J control mice infected with lymphocytic choroid plexitis virus (LCMV) were tested for T cell proliferation, differentiation and function on the 8th day after infection. Model 2: adoptive transfer could specifically recognize the SMARTA or P14 T cells of TCR transgenic mice with LCMV to Fgl2 knockout mice (experimental group) and C57BL/6J control mice on the 8th day after LCMV infection. The proliferation and differentiation of donor SMARTA or P14 cells in mice were detected. Results the expression of FGL2 was up-regulated during acute LCMV infection (P0.05), and there was no significant difference in the frequency and number of T _ (FH) T cells (T _ (FH) Th1) and effector CD8~ T cells between the experimental group and the control group (P0.05). There was no significant difference in the titer of heterosexual antibodies and the frequency of expression of TNF- 伪 and CD107a/b in CD8~ T cells (P0.05). Conclusion FGL2 does not affect the immune response of T cells during acute viral infection.
【作者单位】：第三军医大学全军免疫学研究所;
【基金】：国家自然科学基金(31470870)
【分类号】：R392

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