小鼠舌菌状乳头及味蕾发育过程中组织形态变化及Shh的表达

发布时间：2018-08-27 10:22

【摘要】：背景:哺乳动物的舌背黏膜表面与味觉相关的主要是菌状乳头和轮廓乳头。味觉异常严重影响人们的生活质量,因此了解菌状乳头及其味蕾的发育过程,对于解决因其异常导致的味觉减退有重要意义。目的:观察小鼠舌菌状乳头及味蕾的发生、发育的组织形态变化。方法:取C57BL/6J小鼠胚胎期13.5-18.5 d(E13.5-E18.5),出生后0.5 d(PN0.5),PN4.5,PN7.5,PN14.5,PN21.5,PN90.5及出生后1.5年(PN1.5Y)的舌标本,进行扫描电镜观察及苏木精-伊红染色观察,同时使用Shh抗体进行免疫组织化学染色。取E16.5和E18.5小鼠舌标本进行S-100的免疫组织化学染色。结果与结论:(1)菌状乳头数量的决定主要发生于E13.5-E14.5。从E15.5开始,菌状乳头形态上逐渐发育成熟。味蕾发育开始于E16.5,至PN7.5味孔开始形成。成年期和老年期味蕾细胞和细胞间质减少,并可见到发生于菌状乳头周围的角化;(2)Shh在E14.5表达于菌状乳头的上皮细胞,E16.5-E18.5表达于菌状乳头顶端的上皮细胞,并且在成年期和老年期的味蕾细胞都有所表达。说明Shh在菌状乳头和味蕾发生、发育、成熟及维持的过程中发挥着重要作用;(3)在E18.5,S-100表达于菌状乳头下方的结缔组织,说明神经长入参与了味蕾发育完善成熟的过程。
[Abstract]:Background: the mucosal surface of the dorsal tongue of mammals is mainly associated with the taste of bacillary papilla and outline papilla. Taste abnormality seriously affects people's quality of life, so it is important to understand the development process of mycelial nipples and taste buds in order to solve the hypothyroidism caused by abnormal taste nipples. Objective: to observe the development and histological changes of tongue papilla and taste buds in mice. Methods: the tongue specimens of C57BL/6J mice were collected from embryonic stage 13.5-18.5 days (E13.5-E18.5), postnatal 0.5 day (PN0.5) PN4.5 and PN14.5PN21.5PN90.5 and 1.5 years postnatal (PN1.5Y). The samples were observed by scanning electron microscope and hematoxylin eosin staining. Meanwhile, Shh antibody was used for immunohistochemical staining. The tongue specimens of E16.5 and E18.5 mice were stained with S-100 immunohistochemical staining. Results and conclusions: (1) the determination of the number of bacteriform papilla mainly occurred in E13.5-E14.5. From E15. 5, the mycelial papilla developed and matured in shape. Taste buds began to develop in E 16. 5 and began to form taste holes in PN7.5. In adult and old age, taste bud cells and interstitial cells decreased, and keratosis occurred around the mycelial papilla. (2) Shh was expressed in the epithelial cells of E14.5-E18.5 at the apical end of the myceliform papilla. And both in adulthood and in old age, taste bud cells are expressed. It is suggested that Shh plays an important role in the development, maturation and maintenance of the papilla and taste buds. (3) the connective tissue under the myceliform papilla expressed in E18.5T S-100, which indicates that the nerve growth takes part in the process of taste bud development and maturation.
【作者单位】：首都医科大学附属北京友谊医院口腔科;首都医科大学附属北京友谊医院病理科;
【基金】：国家自然科学基金项目(81170943) 北京市自然科学基金(7162053)~~
【分类号】：R339.13

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