陈皮及其主要活性成分对脾虚模型大鼠血清胃泌素、血浆乙酰胆碱、P物质、胃动素和血管活性肠肽的影响
发布时间：2018-09-05 15:38
【摘要】：目的:考察陈皮及其主要活性成分对脾虚模型大鼠血清胃泌素(GAS)、血浆乙酰胆碱(ACh)、P物质(SP)、胃动素(MTL)和血管活性肠肽(VIP)的影响。方法:以苦寒泻下加饥饱失常法复制大鼠脾虚模型,陈皮水煎液、橙皮苷、辛弗林及4个单体组合物(橙皮苷、辛弗林、川陈皮素、橘皮素)分别灌胃给药,采用ELISA法检测血清GAS、血浆ACh、SP、MTL和VIP的含量变化,考察陈皮水煎液及其主要活性成分对脾虚模型大鼠的影响。结果:模型组大鼠ACh、GAS、MTL和SP含量较空白对照组明显降低,而VIP含量则显著增加。给药后,吗丁啉6 mg/kg组GAS和MTL水平较模型组显著升高;橙皮苷12.68 mg/kg剂量组血清中GAS含量与模型组比较显著增加;橙皮苷12.68、25.36 mg/kg剂量组血浆中ACh含量,6.34 mg/kg剂量组血浆中SP含量,6.34、12.68、25.36 mg/kg剂量组血浆中MTL含量以及6.34 mg/kg剂量组血浆中VIP含量较模型组明显降低。辛弗林8.4 mg/kg剂量组血浆中ACh和MTL水平较模型组显著增加;辛弗林2.1 mg/kg剂量组血浆中SP含量和8.4 mg/kg剂量组血浆中VIP含量与模型组比较明显降低。4个单体组合物46.36 mg/kg剂量组血清中GAS、血浆中ACh以及SP含量较模型组比较显著增加;4个单体组合物11.59mg/kg剂量组血清中GAS、血浆中ACh、SP含量以及四个单体组合物11.59、23.18、46.36 mg/kg剂量组血浆中MTL含量与模型组比较明显降低。陈皮水煎液1.05 g/kg剂量组血清中GAS含量,4.2 g/kg剂量组血浆中ACh和SP含量与模型组比较明显增加;陈皮水煎液1.05、2.1 g/kg剂量组血浆中MTL和VIP含量较模型组明显降低。结论:橙皮苷和辛弗林是陈皮的药效物质。橙皮苷可能通过升高GAS的含量和抑制ACh、SP、MTL和VIP的分泌来促胃肠运动;辛弗林可能通过增加ACh和MTL的分泌和降低SP和VIP的含量来促胃肠运动;陈皮可能通过升高ACh、GAS和SP的含量和抑制MTL和VIP的分泌来促胃肠运动,这可能是陈皮促胃肠动力的机制之一。
[Abstract]:Aim: to investigate the effects of pericarp and its main active components on serum gastrin (GAS), plasma acetylcholine (ACh) motilin (SP),) motilin (MTL) and vasoactive intestinal peptide (VIP) (VIP) in rats with spleen deficiency. Methods: the spleen asthenia model of rats was induced by cold diarrhea and hunger and satiety. The decoction of pericarp, hesperidin, Simverin and four monomers (hesperidin, Simverin, Chuan Chen Pei, Orange Peel) were administered intragastrically respectively. The changes of plasma ACh,SP,MTL and VIP in serum GAS, were detected by ELISA method, and the effects of the decoction and its main active components on the spleen deficiency model rats were investigated. Results: the content of ACh,GAS,MTL and SP in the model group was significantly lower than that in the control group, while the content of VIP in the model group was significantly higher than that in the control group. After administration, the levels of GAS and MTL in morpholine 6 mg/kg group were significantly higher than those in model group, and the serum GAS content in hesperidin 12.68 mg/kg group was significantly higher than that in model group. Content of ACh in plasma of the dose group of 12. 68 ~ 25. 36 mg/kg the content of SP in the plasma of the 6. 34 mg/kg group was significantly lower than that of the model group. The content of MTL in the plasma of the dose group of 6. 34 mg/kg and the content of VIP in the plasma of the dose of 6. 34 mg/kg were significantly lower than that of the model group. The levels of plasma ACh and MTL in the Simferine 8.4 mg/kg group were significantly higher than those in the model group. Compared with model group, the plasma SP content of Simferine 2.1 mg/kg dose group and the plasma VIP content of 8.4 mg/kg dose group were significantly lower than those of model group. The ACh and SP contents in serum of four monomer compositions of 46.36 mg/kg group were significantly higher than those of model group. The content of ACh,SP in serum of the four monomeric compositions (11.59mg/kg) and of the four monomer compositions (11.59 ~ 23.186.36 mg/kg) was significantly lower than that of the model group (P < 0.05), and the content of ACh,SP in the plasma of the four monomeric compositions was significantly lower than that of the model group. The contents of serum GAS and SP in serum of the group of 1.05 g/kg decoction and 4.2 g/kg were significantly higher than those of the model group, and the contents of MTL and VIP in the group of 1.05 ~ 2.1 g/kg were significantly lower than those in the model group. Conclusion: hesperidin and Simverin are the effective substances of pericarp. Hesperidin may promote gastrointestinal motility by increasing the content of GAS and inhibiting the secretion of ACh,SP,MTL and VIP, Simverin may promote gastrointestinal motility by increasing the secretion of ACh and MTL and decreasing the contents of SP and VIP. Pericarp may promote gastrointestinal motility by increasing the content of ACh,GAS and SP and inhibiting the secretion of MTL and VIP, which may be one of the mechanisms of promoting gastrointestinal motility.
【作者单位】： 江西中医药大学现代中药制剂教育部重点实验室;
【基金】：国家自然科学基金项目(81460575,81260612) 江西中医药大学校级研究生创新项目(JZYC15S43)
【分类号】：R285.5;R-332
[Abstract]:Aim: to investigate the effects of pericarp and its main active components on serum gastrin (GAS), plasma acetylcholine (ACh) motilin (SP),) motilin (MTL) and vasoactive intestinal peptide (VIP) (VIP) in rats with spleen deficiency. Methods: the spleen asthenia model of rats was induced by cold diarrhea and hunger and satiety. The decoction of pericarp, hesperidin, Simverin and four monomers (hesperidin, Simverin, Chuan Chen Pei, Orange Peel) were administered intragastrically respectively. The changes of plasma ACh,SP,MTL and VIP in serum GAS, were detected by ELISA method, and the effects of the decoction and its main active components on the spleen deficiency model rats were investigated. Results: the content of ACh,GAS,MTL and SP in the model group was significantly lower than that in the control group, while the content of VIP in the model group was significantly higher than that in the control group. After administration, the levels of GAS and MTL in morpholine 6 mg/kg group were significantly higher than those in model group, and the serum GAS content in hesperidin 12.68 mg/kg group was significantly higher than that in model group. Content of ACh in plasma of the dose group of 12. 68 ~ 25. 36 mg/kg the content of SP in the plasma of the 6. 34 mg/kg group was significantly lower than that of the model group. The content of MTL in the plasma of the dose group of 6. 34 mg/kg and the content of VIP in the plasma of the dose of 6. 34 mg/kg were significantly lower than that of the model group. The levels of plasma ACh and MTL in the Simferine 8.4 mg/kg group were significantly higher than those in the model group. Compared with model group, the plasma SP content of Simferine 2.1 mg/kg dose group and the plasma VIP content of 8.4 mg/kg dose group were significantly lower than those of model group. The ACh and SP contents in serum of four monomer compositions of 46.36 mg/kg group were significantly higher than those of model group. The content of ACh,SP in serum of the four monomeric compositions (11.59mg/kg) and of the four monomer compositions (11.59 ~ 23.186.36 mg/kg) was significantly lower than that of the model group (P < 0.05), and the content of ACh,SP in the plasma of the four monomeric compositions was significantly lower than that of the model group. The contents of serum GAS and SP in serum of the group of 1.05 g/kg decoction and 4.2 g/kg were significantly higher than those of the model group, and the contents of MTL and VIP in the group of 1.05 ~ 2.1 g/kg were significantly lower than those in the model group. Conclusion: hesperidin and Simverin are the effective substances of pericarp. Hesperidin may promote gastrointestinal motility by increasing the content of GAS and inhibiting the secretion of ACh,SP,MTL and VIP, Simverin may promote gastrointestinal motility by increasing the secretion of ACh and MTL and decreasing the contents of SP and VIP. Pericarp may promote gastrointestinal motility by increasing the content of ACh,GAS and SP and inhibiting the secretion of MTL and VIP, which may be one of the mechanisms of promoting gastrointestinal motility.
【作者单位】： 江西中医药大学现代中药制剂教育部重点实验室;
【基金】：国家自然科学基金项目(81460575,81260612) 江西中医药大学校级研究生创新项目(JZYC15S43)
【分类号】：R285.5;R-332
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