GM-CSF浓度梯度对树突状细胞体外诱导培养的影响
[Abstract]:Aim: to observe the effects of granulocyte-macrophage colony stimulating factor (granulocyte-macrophage colonystimulating factor,GM-CSF) combined with interleukin-4 (interleukin,IL-4) on the morphology, purity and cell number of murine bone marrow-derived dendritic cells (dendritic cell,DC), and to explore the optimal conditions for the induction and culture of dendritic cells. Methods: the mouse bone marrow mononuclear cells were isolated and purified, and the morphologic and phenotypic observation and activation of allogeneic lymphocytes were performed by adding different concentration gradient GM-CSF and quantitative IL-4, respectively. Results: the number, purity and maturity of bone marrow-derived dendritic cells increased with the increase of GM-CSF concentration in a certain concentration range. Too high concentration of GM-CSF resulted in inhibitory effect. Therefore, it can be inferred that the ideal range of GM-CSF concentration should be 10-20 ng/mL.. Conclusion: the suitable concentration of GM-CSF combined with IL-4 can enhance the ability of mouse bone marrow mononuclear cells to differentiate into dendritic cells. The rational use of two kinds of cytokines can increase the number, purity and maturity of dendritic cells.
【作者单位】: 昆明医科大学第二附属医院肝胆胰外科一病区;
【基金】:教育部博士点新教师基金资助项目,No.201053171200022010 年度云南省自然科学基金资助项目,No.2010ZC127~~
【分类号】:R392
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